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Vaccine Design pp 351–364Cite as

Generation of Lymphocytic Choriomeningitis Virus Based Vaccine Vectors

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1404))

Abstract

Vaccination with a recombinant LCMV based vector expressing tumor-associated or viral antigens is a safe and versatile method to induce an immune response against tumors or viral infections. Here, we describe the generation of recombinant LCMV vectors in which the gene encoding the viral LCMV-GP was substituted with a gene of interest (vaccine antigen). This renders the vaccine vector propagation-incompetent while it preserves the property of eliciting a strong cytotoxic T cell response.

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Author information

Authors and Affiliations

  1. Institute of Immunobiology, Kantonsspital St. Gallen, Rorschacher Str. 95, St. Gallen, 9007, Switzerland

    Sandra Ring & Lukas Flatz

Authors
  1. Sandra Ring
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  2. Lukas Flatz
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Corresponding author

Correspondence to Lukas Flatz .

Editor information

Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, PA, USA

    Sunil Thomas

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© 2016 Springer Science+Business Media New York

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Ring, S., Flatz, L. (2016). Generation of Lymphocytic Choriomeningitis Virus Based Vaccine Vectors. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 1404. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-3389-1_24

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  • DOI: https://doi.org/10.1007/978-1-4939-3389-1_24

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-3388-4

  • Online ISBN: 978-1-4939-3389-1

  • eBook Packages: Springer Protocols

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