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Sickle Cell Imaging Flow Cytometry Assay (SIFCA)

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Imaging Flow Cytometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1389))

Abstract

Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.

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References

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Acknowledgements

This work was supported by the National Heart, Lung and Blood Division of Intramural Research (1 ZIA HL006013-03 and 1 ZIA HL006149 01). The authors would like to acknowledge James Nichols, RN for blood sample procurement, and Xunde Wang, Ph.D., for assistance with the glovebox figure.

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Authors and Affiliations

  1. Department of Clinical Pathology, School of Medical Sciences, University of Campinas, Campinas, Brazil

    Kleber Y. Fertrin

  2. Hematology and Hemotherapy Center—Hemocentro Campinas, University of Campinas, Campinas, Brazil

    Kleber Y. Fertrin

  3. NHLBI Flow Cytometry Core Facility, NHLBI, NIH, Building 31, Room 5A52, MSC 2486, 31 Center Drive, Bethesda, MD, 20892, USA

    Leigh Samsel & J. Philip McCoy Jr.

  4. Department of Clinical Hematology, Academic Medical Centre, Amsterdam, The Netherlands

    Eduard J. van Beers

  5. Hematology Branch, NHLBI, NIH, Bethesda, MD, 20892, USA

    Laurel Mendelsohn

  6. Division of Hematology/Oncology, Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, 15261, USA

    Gregory J. Kato

Authors
  1. Kleber Y. Fertrin
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  2. Leigh Samsel
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  3. Eduard J. van Beers
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  4. Laurel Mendelsohn
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  5. Gregory J. Kato
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  6. J. Philip McCoy Jr.
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Corresponding author

Correspondence to J. Philip McCoy Jr. .

Editor information

Editors and Affiliations

  1. Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, Massachusetts, USA

    Natasha S. Barteneva

  2. A.N. Belozersky Institute of Physico-Che, M.V. Lomonosov Moscow State University, Moscow, Russia

    Ivan A. Vorobjev

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© 2016 Springer Science+Business Media New York

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Fertrin, K.Y., Samsel, L., van Beers, E.J., Mendelsohn, L., Kato, G.J., McCoy, J.P. (2016). Sickle Cell Imaging Flow Cytometry Assay (SIFCA). In: Barteneva, N., Vorobjev, I. (eds) Imaging Flow Cytometry. Methods in Molecular Biology, vol 1389. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3302-0_20

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  • DOI: https://doi.org/10.1007/978-1-4939-3302-0_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3300-6

  • Online ISBN: 978-1-4939-3302-0

  • eBook Packages: Springer Protocols

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