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Imaging Flow Cytometry pp 195–205Cite as

Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1389))

Abstract

Phenotypic assays are increasingly employed to provide clues about drug mechanisms. In antimalarial drug screening, however, the majority of assays are designed to only measure parasite-killing activity. We describe here a high-content assay to detect drug-mediated perturbation of the digestive vacuole integrity in the trophozoite stage of Plasmodium falciparum, using the ImageStream imaging flow cytometer.

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Author information

Authors and Affiliations

  1. Department of Microbiology, National University of Singapore, Blk MD4, Level 3, 5 Science Drive 2, Singapore, 117545, Singapore

    Yan Quan Lee & Kevin S. W. Tan

  2. NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore

    Yan Quan Lee & Kevin S. W. Tan

  3. EMD Millipore, 645 Elliott Ave West, Suite 100, Seattle, WA, 98119, USA

    Brian E. Hall

Authors
  1. Yan Quan Lee
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  2. Brian E. Hall
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  3. Kevin S. W. Tan
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Corresponding author

Correspondence to Kevin S. W. Tan .

Editor information

Editors and Affiliations

  1. Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, Massachusetts, USA

    Natasha S. Barteneva

  2. A.N. Belozersky Institute of Physico-Che, M.V. Lomonosov Moscow State University, Moscow, Russia

    Ivan A. Vorobjev

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Lee, Y.Q., Hall, B.E., Tan, K.S.W. (2016). Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry. In: Barteneva, N., Vorobjev, I. (eds) Imaging Flow Cytometry. Methods in Molecular Biology, vol 1389. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3302-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3302-0_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3300-6

  • Online ISBN: 978-1-4939-3302-0

  • eBook Packages: Springer Protocols

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