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Plant Cell Division pp 51–57Cite as

Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1370))

Abstract

This chapter focuses on visualization of cell cycle progression in plant tissues using the dual-color marker system “Cytrap.” The Cytrap line carries a part of Arabidopsis CDT1a fused to the red fluorescent protein (RFP) gene, which monitors the cell cycle phases from S to late G2 or early mitosis, and the G2/M-specific cyclin B1 marker fused to the green fluorescent protein (GFP) gene. We introduce growth conditions of Arabidopsis roots, the setup for microscopic observation, and analysis of obtained images.

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Author information

Authors and Affiliations

  1. Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan

    Shiori Sugamata Aki & Masaaki Umeda

  2. JST, CREST, Ikoma, Nara, 630-0192, Japan

    Shiori Sugamata Aki & Masaaki Umeda

Authors
  1. Shiori Sugamata Aki
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  2. Masaaki Umeda
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Corresponding author

Correspondence to Masaaki Umeda .

Editor information

Editors and Affiliations

  1. Institut National de la Recherche Agronomique, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, Laboratoire de Reproduction et Développement des Plantes, Centre National de la Recherche Scientifique, Lyon, France

    Marie-Cécile Caillaud

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Cite this protocol

Aki, S.S., Umeda, M. (2016). Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis . In: Caillaud, MC. (eds) Plant Cell Division. Methods in Molecular Biology, vol 1370. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3142-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-3142-2_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3141-5

  • Online ISBN: 978-1-4939-3142-2

  • eBook Packages: Springer Protocols

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