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Production, Isolation, and Structural Analysis of Ligands and Receptors of the TGF-β Superfamily

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Book cover TGF-β Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1344))

Abstract

The ability to understand the molecular mechanisms by which secreted signaling proteins of the TGF-β superfamily assemble their cell surface receptors into complexes to initiate downstream signaling is dependent upon the ability to determine atomic-resolution structures of the signaling proteins, the ectodomains of the receptors, and the complexes that they form. The structures determined to date have revealed major differences in the overall architecture of the signaling complexes formed by the TGF-βs and BMPs, which has provided insights as to how they have evolved to fulfill their distinct functions. Such studies, have however, only been applied to a few members of the TGF-β superfamily, which is largely due to the difficulty of obtaining milligram-scale quantities of highly homogenous preparations of the disulfide-rich signaling proteins and receptor ectodomains of the superfamily. Here we describe methods used to produce signaling proteins and receptor ectodomains of the TGF-β superfamily using bacterial and mammalian expression systems and procedures to purify them to homogeneity.

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Acknowledgements

The author would also like to acknowledge the funding agencies that have supported the TGF-β research underway in his laboratory, including the NIH (GM58670 and CA172886), the Robert A. Welch Foundation (AQ-1842), and the Cancer Prevention and Research Institute in Texas (RP120867).

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Authors and Affiliations

  1. Protein Chemistry, Novo Nordisk Research Center China, 20 Life Science Park Rd, Bldg 2, Beijing, 102206, China

    Tao Huang & Andrew P. Hinck

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  1. Tao Huang
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  2. Andrew P. Hinck
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Correspondence to Andrew P. Hinck .

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Editors and Affiliations

  1. Departments of Surgery and Molecular & Cellular Biology, Baylor College of Medicine, Houston, Texas, USA

    Xin-Hua Feng

  2. Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang Proviince, China

    Pinglong Xu

  3. Department of Surgery, Baylor College of Medicine, Houston, Texas, USA

    Xia Lin

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Huang, T., Hinck, A.P. (2016). Production, Isolation, and Structural Analysis of Ligands and Receptors of the TGF-β Superfamily. In: Feng, XH., Xu, P., Lin, X. (eds) TGF-β Signaling. Methods in Molecular Biology, vol 1344. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2966-5_4

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  • DOI: https://doi.org/10.1007/978-1-4939-2966-5_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2965-8

  • Online ISBN: 978-1-4939-2966-5

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