Abstract
In contrast to planktonic cultures of the human fungal pathogen Candida albicans, C. albicans biofilms can contain a persister subpopulation that is tolerant to high concentrations of currently used antifungals. In this chapter, the method to determine the persister fraction in a C. albicans biofilm treated with an antifungal compound is described. To this end, a mature biofilm is developed and subsequently treated with a concentration series of the antifungal compound of interest. Upon incubation, the fraction of surviving biofilm cells is determined by plating and plotted versus the used concentrations of the antifungal compound. If a persister subpopulation in the biofilm is present, the dose-dependent killing of the biofilm cells results in a biphasic killing pattern.
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Acknowledgements
The research leading to these results has received funding from the European Commission’s Seventh Framework Programme (FP7/2007-2013) under the grant agreement COATIM (project n° 278425).
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De Brucker, K., De Cremer, K., Cammue, B.P.A., Thevissen, K. (2016). Protocol for Determination of the Persister Subpopulation in Candida Albicans Biofilms. In: Michiels, J., Fauvart, M. (eds) Bacterial Persistence. Methods in Molecular Biology, vol 1333. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2854-5_6
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DOI: https://doi.org/10.1007/978-1-4939-2854-5_6
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2853-8
Online ISBN: 978-1-4939-2854-5
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