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Grid-Immunoblotting

  • Protocol
Western Blotting

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1312))

Abstract

Grid-immunoblotting is a fast, simple, and efficient method for simultaneously testing multiple allergens utilizing small amount of antibody.

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References

  1. Reese G, Schmechel D, Ayuso R, Lehrer SB (2001) Grid-immunoblotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross-reactivity. J Chromatogr B 756:151–156

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  2. Yeliosof O, Scofield RH (2009) Grid-immunoblotting. Methods Mol Biol 536:289–292

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  3. Kurien BT, Scofield RH (2003) Protein blotting: a review. J Immunol Methods 274:1–15

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  4. Demeulemester C, Peltre G, Laurente M, Panheleux D, David B (1987) Cyanogen bromide-activated nitrocellulose membranes: a new tool for immunoprint techniques. Electrophoresis 8:71

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Author information

Authors and Affiliations

  1. Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA

    Olga Yeliosof B.S. & R. Hal Scofield M.D.

  2. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA

    R. Hal Scofield M.D.

  3. Department of Veterans Affairs Medical Center, Oklahoma City, OK, 73104, USA

    R. Hal Scofield M.D.

  4. Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, 825 NE 13th Street, Mailstop #24, Oklahoma City, OK, 73104, USA

    R. Hal Scofield M.D.

Authors
  1. Olga Yeliosof B.S.
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  2. R. Hal Scofield M.D.
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Corresponding author

Correspondence to R. Hal Scofield M.D. .

Editor information

Editors and Affiliations

  1. Department of Medicine, University of Oklahoma, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Department of Medicine, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Yeliosof, O., Scofield, R.H. (2015). Grid-Immunoblotting. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_37

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  • DOI: https://doi.org/10.1007/978-1-4939-2694-7_37

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2693-0

  • Online ISBN: 978-1-4939-2694-7

  • eBook Packages: Springer Protocols

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