Editors:
- Includes cutting-edge methods and protocols in plant phosphoproteomics
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1306)
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Table of contents (17 protocols)
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Front Matter
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Back Matter
About this book
This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein and a large central vacuole leading to low protein yield and increased degradative enzyme activity. The methodologies in this book seek to move beyond these issues. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Plant Phosphoproteomics: Methods and Protocols serves as an ideal reference for researchers investigating this vital area of plant science.
Editors and Affiliations
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Department of Plant Systems Biology, Universität Hohenheim, Stuttgart, Germany
Waltraud X. Schulze
Bibliographic Information
Book Title: Plant Phosphoproteomics
Book Subtitle: Methods and Protocols
Editors: Waltraud X. Schulze
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2648-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2647-3Published: 01 May 2015
Softcover ISBN: 978-1-4939-4937-3Published: 09 October 2016
eBook ISBN: 978-1-4939-2648-0Published: 30 April 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 231
Number of Illustrations: 8 b/w illustrations, 16 illustrations in colour
Topics: Plant Sciences, Proteomics