Abstract
Pulsed-field gel electrophoresis (PFGE) has been extensively used to estimate the genetic diversity of Clostridium botulinum. In addition, PFGE is the standard method for investigating foodborne outbreaks associated with various enteric pathogens, including C. botulinum. PFGE can be used to exclude a suspected but not confirmed food source when the patterns of the food and clinical isolates are different. Indistinguishable PFGE patterns may also be useful for linking isolates between patients or to a food source, but results must be interpreted within an epidemiological context to ensure isolates are truly related. Here, we describe a standardized laboratory protocol for molecular subtyping of C. botulinum by PFGE.
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Lúquez, C., Joseph, L.A., Maslanka, S.E. (2015). Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis. In: Jordan, K., Dalmasso, M. (eds) Pulse Field Gel Electrophoresis. Methods in Molecular Biology, vol 1301. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2599-5_10
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DOI: https://doi.org/10.1007/978-1-4939-2599-5_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2598-8
Online ISBN: 978-1-4939-2599-5
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