Microfluidic and Compartmentalized Platforms for Neurobiological Research

1. Front Matter

   Pages i-xiii

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2. Designs and Methods

   1. Front Matter

      Pages 1-1

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   2. The Fabrication of Microfluidic Platforms with Pneumatically/Hydraulically Controlled PDMS Valves and Their Use in Neurobiological Research

      • Bryson M. Brewer, Donna J. Webb, Deyu Li

      Pages 3-23

   3. A Reliable Reversible Bonding Method for Perfused Microfluidic Devices

      • Paola Occhetta, Emilia Biffi, Marco Rasponi

      Pages 25-38

   4. Bridging Two Cultures: Minimalistic Networks Prepared by Microfluidic Arraying, and Open Access Compartments for Electrophysiology

      • Jonathan West, Ngoc-Duy Dinh, Heike Hardelauf, Ya-Yu Chiang, Tracey A. Newman, Mariana Vargas-Caballero et al.

      Pages 39-56

3. Axonal Guidance and Manipulation

   1. Front Matter

      Pages 57-57

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   2. Asymmetric Genetic Manipulation and Patch Clamp Recording of Neurons in a Microfluidic Chip

      • Prasanna Sakha, Cecilia Brunello, Joonas Heikkinen, Ville Jokinen, Henri J. Huttunen

      Pages 59-81

   3. Development of a Compartmentalized Biochip for Axonal Isolation and Neuronal-Circuit Formation at the Single-Cell Level

      • Ting Huang, Russell K. Pirlo, Wan Qin, Yongliang Lin, Lina Wei, Lucas Schmidt et al.

      Pages 83-104

   4. Campenot Cultures and Microfluidics Provide Complementary Platforms for Spatial Study of Dorsal Root Ganglia Neurons

      • Sara J. Fenstermacher, Maria F. Pazyra-Murphy, Rosalind A. Segal

      Pages 105-124

4. Manipulation of Synapses

   1. Front Matter

      Pages 125-125

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   2. Development of Microfluidic Devices for the Manipulation of Neuronal Synapses

      • Anika Jain, Martha U. Gillette

      Pages 127-137

   3. Use of a 3-Compartment Microfluidic Device to Study Activity Dependent Synaptic Competition

      • Ainsley Coquinco, Max Cynader

      Pages 139-146

5. Interaction of Different Cell Populations

   1. Front Matter

      Pages 147-147

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   2. Multi-compartment Neuron–Glia Coculture Microsystem

      • Jaewon Park, Sunja Kim, Jianrong Li, Arum Han

      Pages 149-159

   3. Compartmentalized Microfluidic Platforms as Tool of Choice to Study the Interaction Between Neurons and Osteoblasts

      • Estrela Neto, Diogo Paramos-de-Carvalho, Ana Henriques Lourenço, Paulo Aguiar, Meriem Lamghari

      Pages 161-179

   4. A Novel In Vitro Primary Culture Model of the Lower Motor Neuron–Neuromuscular Junction Circuit

      • Katherine A. Southam, Anna E. King, Catherine A. Blizzard, Graeme H. McCormack, Tracey C. Dickson

      Pages 181-193

6. Pharmacological Manipulation and Screening

   1. Front Matter

      Pages 195-195

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   2. Compartmentalized Microfluidics for In Vitro Alzheimer’s Disease Studies

      • Yufei Ren, Anja Kunze, Philippe Renaud

      Pages 197-215

   3. Selective Biochemical Manipulation of Twin Neuronal Networks on Microelectrode Arrays

      • Giulia Regalia, Emilia Biffi, Marco Rasponi, Alessandra Pedrocchi

      Pages 217-230

   4. Compartmentalized Synapse Microarray for High-Throughput Screening

      • Amol D. Jadhav, Wei Li, Zhen Xu, Peng Shi

      Pages 231-245

This volume presents cutting-edge techniques to design, fabricate and use compartmentalized microfluidic devices. Chapters encompass axon guidance and manipulation, compartmentalized devices for synapse manipulation, cell populations interaction either in physiological or pathological condition and compartmentalized devices for pharmacological research and drug discovery. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Authoritative and practical, Microfluidic and Compartmentalized Platforms for Neurobiological Research provides practical skills needed to fabricate, use microfluidics and compartmentalized platforms with neuronal cell cultures as well as the strengths of these exciting devices and their precious contribution in the field of Neuroscience.

• arraying neurons
• cell populations
• drug discovery
• electrophysiology recordings
• hydraulically controlled valves
• magnetic forces
• microfluidic chip
• neuron-glia co-culture microsystem platform
• pneumatically controlled valves
• synapse manipulation

• Bioengineering Laboratory, Department of Electronics, Information and Bioengineering, Scientific Institute IRCCS E. Medea, Bosisio Parini (LC), Milano, Italy

  Emilia Biffi

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