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Mouse Models of Cancer pp 321–348Cite as

Tumor Imaging Technologies in Mouse Models

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1267))

Abstract

In this chapter, we describe protocols for tumor imaging technologies in mouse models. These models utilize human cancer cell lines which have been genetically engineered to selectively express high levels of green fluorescent protein (GFP) or red fluorescent protein (RFP). Tumors with fluorescent genetic reporters are established subcutaneously in nude mice, and fragments of the subcutaneous tumors are then surgically transplanted onto the orthotopic organ. Locoregional tumor growth and distant metastasis of these orthotopic implants occur spontaneously and rapidly throughout the abdomen in a manner consistent with clinical human disease. Highly specific, high-resolution, real-time quantitative fluorescence imaging of tumor growth and metastasis may be achieved in vivo without the need for contrast agents, invasive techniques, or expensive imaging equipment. Transplantation of RFP-expressing tumor fragments onto the pancreas of GFP- or cyan fluorescent protein (CFP)-expressing transgenic nude mice was used to facilitate visualization of tumor–host interaction between the pancreatic cancer cells and host-derived stroma and vasculature. Such in vivo models have enabled us to visualize in real time and acquire images of the progression of pancreatic cancer in the live animal, and to demonstrate the real-time antitumor and antimetastatic effects of several novel therapeutic strategies on a variety of malignancies. We discuss studies from our laboratory that demonstrate that fluorescence imaging in mice is complementary to other modalities such as magnetic resonance imaging (MRI) or ultrasound. These fluorescent models are powerful and reliable tools with which to investigate metastatic human cancer and novel therapeutic strategies directed against it.

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Acknowledgments

This work was supported in part by grants from the National Cancer Institute CA109949 and CA132971.

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Authors and Affiliations

  1. Department of Surgery, University of California San Diego, Moores Cancer Center, 3855 Health Sciences Drive #0987, La Jolla, CA, 92093-0987, USA

    Michael Bouvet M.D. & Robert M. Hoffman Ph.D.

  2. AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA, 92111, USA

    Robert M. Hoffman Ph.D.

Authors
  1. Michael Bouvet M.D.
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  2. Robert M. Hoffman Ph.D.
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Correspondence to Michael Bouvet M.D. or Robert M. Hoffman Ph.D. .

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Editors and Affiliations

  1. Medical University Vienna (MUV) Institute for Cancer Research, Vienna, Austria

    Robert Eferl

  2. Research (LBI-CR), Medical University Vienna (MUV), Center of Physiology and Pharmacology & Ludwig Boltzmann Institute for Cancer, Vienna, Austria

    Emilio Casanova

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Bouvet, M., Hoffman, R.M. (2015). Tumor Imaging Technologies in Mouse Models. In: Eferl, R., Casanova, E. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 1267. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2297-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-2297-0_16

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