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Plant Cell Expansion pp 1–21Cite as

Penium margaritaceum as a Model Organism for Cell Wall Analysis of Expanding Plant Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1242))

Abstract

The growth of a plant cell encompasses a complex set of subcellular components interacting in a highly coordinated fashion. Ultimately, these activities create specific cell wall structural domains that regulate the prime force of expansion, internally generated turgor pressure. The precise organization of the polymeric networks of the cell wall around the protoplast also contributes to the direction of growth, the shape of the cell, and the proper positioning of the cell in a tissue. In essence, plant cell expansion represents the foundation of development. Most studies of plant cell expansion have focused primarily upon late divergent multicellular land plants and specialized cell types (e.g., pollen tubes, root hairs). Here, we describe a unicellular green alga, Penium margaritaceum (Penium), which can serve as a valuable model organism for understanding cell expansion and the underlying mechanics of the cell wall in a single plant cell.

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Abbreviations

AGP:

Arabinogalactan protein

CBM:

Carbohydrate-binding module

CGA:

Charophycean Green Algae

CLSM:

Confocal laser scanning microscope

HG:

Homogalacturonan

LM:

Light microscope

LRW:

London Resin White

PBS:

Phosphate-buffered saline

PBST:

PBS with 1 % Triton-X

Penium:

Penium margaritaceum

PME:

Pectin methyl esterase

RT:

Room temperature

TEM:

Transmission electron microscopy

VPSEM:

Variable pressure scanning electron microscopy

WFLM:

Wide-field fluorescence microscope

WHS:

Sterile Woods Hole medium supplemented with sterile soil extract (Woods Hole Soil)

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Acknowledgments

The authors thank J. Paul Knox (University of Leeds, UK), Marie-Christine Ralet (Institut National de la Recherche Agronomique, Nantes, France), and Harry J. Gilbert (Newcastle University, UK) for monoclonal antibodies and CBMs. We are grateful to Iben Sørensen and Jocelyn K.C. Rose of Cornell University for rewarding and helpful discussion. This work was funded by The Danish Strategic Research Council, The Danish Council for Independent Research, Technology and Production Sciences, and The Villum Foundation’s Young Investigator Programme. Some techniques described here were derived from research funded by the National Science Foundation (USA) grants, NSF-MCB 0919925, NSF-MRI 0922805, and NSF-MRI 0419131.

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Authors and Affiliations

  1. Department of Plant and Environmental Sciences, Faculty ofScience, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Copenhagen, Denmark

    Maja G. Rydahl, Jonatan U. Fangel, Maria Dalgaard Mikkelsen, I. Elisabeth Johansen, Jesper Harholt, Peter Ulvskov, Bodil Jørgensen & William G. T. Willats

  2. Department of Biology and Skidmore Microscopy Imaging Center, Skidmore College, Saratoga Springs, NY, USA

    Amanda Andreas & David S. Domozych

Authors
  1. Maja G. Rydahl
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  2. Jonatan U. Fangel
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  3. Maria Dalgaard Mikkelsen
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  4. I. Elisabeth Johansen
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  5. Amanda Andreas
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  6. Jesper Harholt
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  7. Peter Ulvskov
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  8. Bodil Jørgensen
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  9. David S. Domozych
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  10. William G. T. Willats
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Correspondence to William G. T. Willats .

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Editors and Affiliations

  1. Departamento de Fisiología Biologia Molecular y Celular (DFBMC), University of Buenos Aires IFIByNE-CONICET, Buenos Aires, Argentina

    José M. Estevez

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Rydahl, M.G. et al. (2015). Penium margaritaceum as a Model Organism for Cell Wall Analysis of Expanding Plant Cells. In: Estevez, J. (eds) Plant Cell Expansion. Methods in Molecular Biology, vol 1242. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1902-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-1902-4_1

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  • Publisher Name: Humana Press, New York, NY

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