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Regulatory B Cells in Mouse Models of Intestinal Inflammation

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Regulatory B Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1190))

Abstract

Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory condition with increasing incidence and prevalence around the world. Although B cells had generally been believed to play a pathogenic role in IBD due to the production of autoantibodies, a growing body of evidence from mouse models suggests the coexistence of pathogenic B cells and regulatory B cells, termed Breg, in this disorder. Since some unique techniques are required to closely study the Breg in gut-associated lymphoid tissues (GALT), we herein describe how to induce colitis in mice and how to analyze the phenotype and function of GALT-specific Breg.

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Acknowledgments

The study has been supported primarily by NIH RO1AI081807 and partially by RO1DK091247.

Author information

Authors and Affiliations

  1. Molecular Pathology Unit, Massachusetts General Hospital, 149 CNY-6024, 13th Street, Charlestown, MA, 02114, USA

    Atsushi Nishida

  2. Gastroenterology Division, Massachusetts General Hospital, 149 CNY-6024, 13th Street, Charlestown, MA, 02114, USA

    Atsushi Nishida

  3. Department of Pathology, Harvard Medical School, Boston, MA, USA

    Atsushi Nishida

  4. Gastrointestinal Unit, Massachusetts General Hospital, 55 Fruit Street, Boston, MA, 02114, USA

    Emiko Mizoguchi

  5. Molecular Pathology Unit, Massachusetts General Hospital, 149 CNY-6024, 13th Street, Charlestown, MA, 02129, USA

    Cindy W. Lau & Atsushi Mizoguchi

  6. Department of Medicine, Harvard Medical School, 25 Shattuck Street, Boston, MA, 02115, USA

    Emiko Mizoguchi

  7. Department of Pathology, Harvard Medical School, 25 Shattuck Street, Boston, MA, 02115, USA

    Cindy W. Lau & Atsushi Mizoguchi

Authors
  1. Atsushi Nishida
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  2. Cindy W. Lau
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  3. Emiko Mizoguchi
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  4. Atsushi Mizoguchi
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Corresponding author

Correspondence to Atsushi Mizoguchi .

Editor information

Editors and Affiliations

  1. Department of Medical and Biological Sciences, University of Udine, Udine, Italy

    Gaetano Vitale

  2. Department of Medical and Biological Sciences, University of Udine, Udine, Italy

    Francesca Mion

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Nishida, A., Lau, C.W., Mizoguchi, E., Mizoguchi, A. (2014). Regulatory B Cells in Mouse Models of Intestinal Inflammation. In: Vitale, G., Mion, F. (eds) Regulatory B Cells. Methods in Molecular Biology, vol 1190. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1161-5_16

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  • DOI: https://doi.org/10.1007/978-1-4939-1161-5_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1160-8

  • Online ISBN: 978-1-4939-1161-5

  • eBook Packages: Springer Protocols

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