Abstract
This article describes analytical methods developed to study the disposition of [3 H]enisoprost, a PGE1 analogue, in man. Solid-phase extraction and HPLRC* were used. These methods were scaled up to allow isolation and purification of urinary metabolites. These were identified by GC-MS of their ME-MO-TMS and PFB-MO-TMS derivatives. The latter provided mol. wt. data from the intense [M-PFB]- ions formed by NCI with ammonia, and the former provided information on sites of metabolism from the characteristic EI fragmentation pathways. The addition of a cholinesterase inhibitor, 2 M pyridostigmine bromide, served to stabilize enisoprost in whole blood immediately after collection; in its absence enisoprost was rapidly degraded to its carboxylic acid metabolite (SC-36067).
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Abbreviations
- HPLRC:
-
high performance liquid radiochromatography
- MS:
-
mass spectrometry (EI, electron-impact mode
- NCI:
-
negative-ion chemical ionization)
- MS:
-
methyl ester
- MO:
-
0-methyloxime [rendered as MoMe in art. #A-1]
- PFB:
-
pentafluorobenzyl
- TMS:
-
trimethylsilyl
- PG:
-
prostaglandin
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© 1988 Springer Science+Business Media New York
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Allan, L.A., Hawkins, A.J., Firth, J., Brownsill, R.D., Steiner, J.A., Vose, C.W. (1988). Analytical Methods for [3H]-Enisoprost, an Anti-Secretory PGE1 Analogue, and its Metabolites. In: Reid, E., Robinson, J.D., Wilson, I.D. (eds) Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular. Methodological Surveys in Biochemistry and Analysis, vol 18 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9424-3_4
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DOI: https://doi.org/10.1007/978-1-4757-9424-3_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9426-7
Online ISBN: 978-1-4757-9424-3
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