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Haploids in Cereal Improvement: Anther and Microspore Culture

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Gene Manipulation in Plant Improvement II

Part of the book series: Stadler Genetics Symposia Series ((SGSS))

Abstract

For years, cereals and monocots in general have been poor responders in in vitro haploid production systems when compared to many dicot crops. The response frequency was low with very strong genotype effects and often large numbers of albino progeny. However, we are now seeing evidence of a breakthrough that can provide embryo frequencies equivalent to those obtained in some Brassicas and other dicot crops. These results are with specific genotypes of barley (Hordeum vulgare L.) (Hunter, 1988; Hunter et al., 1989; Olsen, 1987), where green plant production has increased up to 100 fold. As a result, further improvements in wheat (Triticum aestivum L.) and other cereals will likely follow. In addition, plants have been obtained from isolated microspore culture in barley, wheat, rice (Oryza sativa L.) and corn (Zea mays L.). These cultures open up new possibilities for transformation, mutation and selection in cereals.

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Authors and Affiliations

  1. Crop Science Dept., University of Guelph, Guelph, Ontario, Canada, N1G 2W1

    K. J. Kasha, A. Ziauddin & U.-H. Cho

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  1. K. J. Kasha
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  2. A. Ziauddin
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  3. U.-H. Cho
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  1. USDA-ARS, University of Missouri, Columbia, Missouri, USA

    J. Perry Gustafson

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© 1990 Plenum Press, New York

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Kasha, K.J., Ziauddin, A., Cho, UH. (1990). Haploids in Cereal Improvement: Anther and Microspore Culture. In: Gustafson, J.P. (eds) Gene Manipulation in Plant Improvement II. Stadler Genetics Symposia Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7047-5_11

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  • DOI: https://doi.org/10.1007/978-1-4684-7047-5_11

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