Summary
Human recombinant interleukin 4 (IL-4) and interleukin 7 (IL-7) have been modified with biotin-N-hydroxysuccinimide and used to examine the expression of human IL-4 and IL-7 receptors (R) on activated peripheral blood T cells by flow cytometry. Freshly isolated T cells expressed only a low level of IL-4R which remained unchanged when cells were cultured in the absence of stimuli. In the presence of IL-4, IL-7, phytohemag-glutinin A (PHA) or immobilized CD3 monoclonal antibody the intensity of biotinylated IL-4 staining increased approximately twofold on the majority of cells. A combination of mitogen with either IL-4 or IL-7 caused a considerable increase in IL-4 receptor expression over that seen in the presence of mitogen alone. IL-2 alone failed to induce IL-4R although it was able to cause a significant increase in receptor expression on T cells co-cultured with PHA or CD3.
Freshly isolated T cells expressed high levels of IL-7R, as determined by biotinylated IL-7 binding and flow cytometry, which did not change significantly with culture in medium alone. Stimulation with PHA, Concanavalin A (Con A) or CD3 had little effect on the intensity of staining. In contrast, activation with phorbol ester resulted in a decrease in IL-7R expression. Similarly, in the presence of IL-4 or IL-7, but not IL-2, the intensity of staining with biotinylated IL-7 was lowered. Analysis of purified T-cell populations showed that IL-7R were present, and IL-4R could be induced, on both CD4+ and CD8+ populations.
Analysis of IL-4 receptor expression by this flow cytometric technique was supported by results from 125I-labeled IL-4 binding and by Northern blot analysis of mRNA levels. Taken together, the results of these studies show that the use of biotinylated cytokines and flow cytometry provides a very sensitive method with which to study the expression and regulation of cytokine receptors.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
M. Howard, J. Farrar, M. Hilfiker, B. Johnson, K. Takatsu, T. Hamaoka, and W. E. Paul, Identification of a T cell-derived B cell growth factor distinct from interleukin 2, J. Exp. Med. 155:914 (1982).
K. H. Grabstein, L. S. Park, P. J. Morrissey, H. Sassenfeld, V. Price, D. L. Urdal, and M. B. Widmer, Regulation of murine T cell proliferation by B cell stimulatory factor, J. Immunol. 139:1148 (1987).
J. Hu-Li, E. M. Shevach, J. Mizuguchi, J. Ohara, T. Mosmann, and W. E. Paul, B cell stimulatory factor 1 (interleukin 4) is a potent costimulant for normal resting T lymphocytes, J. Exp. Med. 165:157 (1987).
T. R. Mosmann, M. W. Bond, R. L. Coffman, J. Ohara, and W. E. Paul, T cell and mast cell lines respond to B cell stimulatory factor-1, Proc. Natl. Acad. Sci. USA 83:5654 (1986).
R. Noelle, P. Krammer, J. Ohara, J. W. Uhr, and E. S. Vitetta, Increased expression la antigens on resting B cells: an additional role for B-cell growth factor, Proc. Natl. Acad. Sci. USA 81:6149 (1984).
N. W. Roehm, J. Liebson, A. Zlotnik, J. Kappler, P. Marrack, and J. C. Cambier, Interleukin induced increase in la expression by normal mouse B cells, J. Exp. Med. 160:679 (1984).
T. Defrance, J. P. Aubry, F. Rousset, B. Vanbervliet, J. Y. Bonnefoy, N. Arai, Y. Takebe, T. Yokata, F. Lee, K. Arai, J. deVries, and J. Banchereau, Human interleukin IL-4 induces Fereceptors (CD23) on normal human B lymphocytes, J. Exp. Med. 165:1459 (1987).
S. A. Hudak, S. O. Gollinick, D. H. Conrad, and M. R. Kehry, Murine B cell stimulatory factor 1 (interleukin 4) increases expression of the Fc receptor for IgE on mouse B cells, Proc. Natl. Acad. Sci. USA 84:4666 (1987).
J. G. Shields, R. J. Armitage, B. N. Jamieson, P. C. L. Beverley, and R. E. Callard, Increased expression of surface IgM but not IgD or IgG on human B cells in response to IL-4, Immunol. 66:224 (1989).
E. S. Vitetta, J. Ohara, C. Myers, J. Layton, P. H. Krammer, and W. E. Paul, Serological, biochemical and functional identity of B cell stimulatory factor-1 and B cell differentiation factor for IgGl, J. Exp. Med. 162:1726 (1985).
R. L. Coffman, J. Ohara, M. W. Bond, J. Carty, A. Zlotnik, and W. E. Paul, B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells, J. Immunol. 136:4538 (1988).
L. S. Park, D. Friend, K. Grabstein, and D. L. Urdal, Characterization of the high affinity cell-surface receptor for murine B cell stimulating factor 1, Proc. Natl. Acad. Sci. USA 84: 1669 (1987).
J. Ohara and W. E. Paul, Receptors for B-cell stimulatory factor 1 expressed on cells of haematopoietic lineage, Nature (London) 325:537 (1987).
J. W. Lowenthal, B. E. Castle, J. Christiansen, J. Schreurs, D. Rennick, N. Arai, P. Hoy, Y. Takebe, and M. Howard, Expression of high affinity receptors for murine interleukin 4 (BSF-1) on hemopoietic and non-hemopoietic cells, J. Immunol. 140:456 (1988).
A. E. Namen, A. E. Schmierer, C. J. March, R. W. Overell, L. S. Park, D. L. Urdal, and D. Y. Mochizuki, B cell precursor growth-promoting activity. Purification and characterization of a growth factor active on lymphocyte precursors, J. Exp. Med. 167:988 (1988).
P. J. Morrissey, R. G. Goodwin, R. P. Nordan, D. Anderson, K. H. Grabstein, D. Cosman, J. Sims, S. Lupton, B. Acres, S. G. Reed, D. Mochizuki, J. Eisenman, P. J. Conlon, and A. E. Namen, Recombinant interleukin 7 pre-B growth factor has costimulatory activity on purified mature T cells, J. Exp. Med. 169:707 (1989).
R. J. Armitage, A. E. Namen, H. M. Sassenfeld, and K. H. Grabstein, Regulation of human T-cell proliferation by interleukin 7, J. Immunol. 144:938 (1990).
D. L. Urdal, D. Mochizuki, P. J. Conlon, C. J. March, M. L. Remerowski, J. Eisenman, C. Ramthun, and S. Gillis, Lymphokine purification by reversed phase high performance liquid chromatography, J. Chromatogr. 296:171 (1984).
M. B. Widmer, R. B. Acres, H. M. Sassenfeld, and K. H. Grabstein, Regulation of cytolytic cell populations from human peripheral blood by B cell stimulatory factor 1 (interleukin 4), J. Exp. Med. 166:1447 (1987).
R. G. Goodwin, S. Lupton, A. Schmierer, K. J. Hjerrild, R. Jerzy, W. Clevenger, S. Gillis, D. Cosman, and A. E. Namen, Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells, Proc. Natl. Acad. Sci. USA 84:302 (1989).
L. S. Park, D. Friend, H. M. Sassenfeld, and D. L. Urdal. Characterization of the human B cell stimulatory factor 1 receptor, J. Exp. Med. 166:476 (1987).
S. K. Dower, K. Ozato, and D. M. Segal, The interactions of monoclonal antibodies with MHC class I antigens on mouse spleen cells. I. Analysis of the mechanism of binding, J. Immunol. 120:2027 (1984).
C. J. March, B. Mosley, A. Larsen, D. P. Cerretti, G. Braedt, V. Price, S. Gillis, C. S. Henney, S. R. Kronheim, K. Grabstein, P. J. Conlon, T. P. Hopp, and D. Cosman, Cloning, sequence and expresson of two distinct human interleukin-1 complementary DNA s, Nature 315:641 (1985).
L. M. Hoffman, M. K. Fritsch, and J. Gorski, Probable nuclear precursors of preprolactin mRNA in rat pituitary cells, J. Biol. Chem. 256:2597 (1981).
R. L. Idzerda, C. J. March, B. Mosley, S. D. Lyman, T. Vanden Bos, S. D. Gimpel, W. S. Din, K. H. Grabstein, M. B. Widmer, L. S. Park, D. Cosman, and M. P. Beck-Mann, Human interleukin-4 receptor confers biological responsiveness and defines a novel receptor superfamily, J. Exp. Med. 171:861 (1990).
D. L. Jankovic, M. Gibert, D. Baran, J. Ohara, W. E. Paul, and J. Theze. Activation by IL-2, but not IL-4, up-regulates the expression of the p55 subunit of the IL-2 receptor on IL-2-and IL-4-dependent T cell lines, J. Immunol. 142:3113 (1989).
J. Ohara and W. E. Paul, Up-regulation of interleukin 4/B-cell stimulatory factor 1 expression, Proc. Natl. Acad. Sci. USA 85:8221 (1988).
L. S. Park, D.J. Friend, A. E. Schmierer, S. K. Dower, and A. E. Namen, Murine interleukin-7 receptor: characterization on an IL-7-dependent cell line, J. Exp. Med. (in press).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Plenum Press, New York
About this chapter
Cite this chapter
Armitage, R.J., Ziegler, S.F., Beckmann, M.P., Idzerda, R.L., Park, L.S., Fanslow, W.C. (1991). Expression of Receptors for Interleukin 4 and Interleukin 7 on Human T Cells. In: Gupta, S., Paul, W.E., Cooper, M.D., Rothenberg, E.V. (eds) Mechanisms of Lymphocyte Activation and Immune Regulation III. Advances in Experimental Medicine and Biology, vol 292. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5943-2_14
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5943-2_14
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5945-6
Online ISBN: 978-1-4684-5943-2
eBook Packages: Springer Book Archive