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Effect of Spermine on Transfer RNA and Transfer RNA-Ribosome Interactions

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Progress in Polyamine Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 250))

Abstract

Translation of genetic message requires a coordianted interplay of more than a hundred kinds of macromolecules. Besides the ribosomes, which are multicomponent ribonucleoprotein particles by their own nature, molecules of transfer RNA, aminoacyl-tRNA synthetases, soluble protein factors and mRNA are involved in the process. Ribosomes provide an unspecific stage for the codon-anticodon interaction and catalyze the peptide bond formation. Molecules of tRNA play a crucial and highly specific role in the over-all process: they are recognized by specific aminoacyl-tRNA synthetases to be charged with their cognate amino acids; the resulting aminoacyl-tRNAs are then brought, in the form of ternary complexes with GTP and the elongation factor Tu, to the decoding or A site of the ribo-some. There they are screened for the proper codon-anticodon matching and only the correct aminoacyl-tRNA is allowed to enter the transpeptidation reaction. The resulting peptidyl-tRNA is then translocated to the peptidyl or P site to serve as the peptidyl donor in the next round of translation. The process in its simplified form is shown in Figure 1.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, Yugoslavia

    Željko Kućan, Tatjana Naranda & Ivana Weygand-Durašević

  2. Rugjer Bošković Institute, Bijenička cesta 54, YU-41000, Zagreb, Yugoslavia

    Miroslav Plohl & Vesna Nöthig-Laslo

Authors
  1. Željko Kućan
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  2. Tatjana Naranda
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  3. Miroslav Plohl
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  4. Vesna Nöthig-Laslo
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  5. Ivana Weygand-Durašević
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Editor information

Editors and Affiliations

  1. Institute of Biochemistry of Macromolecules, University of Naples, Naples, Italy

    Vincenzo Zappia

  2. The Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey, Pennsylvania, USA

    Anthony E. Pegg

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© 1988 Plenum Press, New York

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Kućan, Ž., Naranda, T., Plohl, M., Nöthig-Laslo, V., Weygand-Durašević, I. (1988). Effect of Spermine on Transfer RNA and Transfer RNA-Ribosome Interactions. In: Zappia, V., Pegg, A.E. (eds) Progress in Polyamine Research. Advances in Experimental Medicine and Biology, vol 250. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5637-0_47

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  • DOI: https://doi.org/10.1007/978-1-4684-5637-0_47

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5639-4

  • Online ISBN: 978-1-4684-5637-0

  • eBook Packages: Springer Book Archive

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