Abstract
The purpose of the present study was to determine what factors govern cellular reduction of hydrogen peroxide. Both catalase and glutathione (GSH) peroxidase play a role in reducing peroxide, however, it has not been precisely determined to what degree each enzyme is involved. Therefore, we have studied the total capacity of two tumor cell lines to reduce peroxide as well as the contribution of each enzyme.
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References
M. E. Varnes, S. Tuttle, and J. E. Biaglow, The use of a peroxidase electrode to measure the effects of nitro compounds on intact cells, in: “Oxygen Transport to Tissue — IV, ” H. Bicher and D. Bruley, eds., Plenum Publishing Co., New York (1983).
M. E. Varnes, J. E. Biaglow, L. Donahue, and S. Tuttle, Effects of glucose and thiol depletion on chemically—induced peroxide production in mammalian cells, in: “Oxygen Transport to Tissue — VI,” D. Bruley, H. Bicher, and D. Reneau, eds., Plenum Publishing Co., New York (1984).
J. E. Biaglow, E. Clark, E. Epp, M. M.rse—Guadio, M. E. Varnes, and J. Mitchell, Nonprotein thiols and the radiation response of A549 human lung carcinoma cells, Int.J.Radiat. Biol. 44: 489 (1983).
YSI Instrument Co., Instructions for YSI Model 25 Oxidase Meter and YSI 2510 Oxidase Probe (1984).
A. Habeeb, Reactions of protein sulfhydryl groups with Eliman’s reagent, Methods in Enz. 25: 457 (1972).
F. Tietze, Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues, Anal. Biochem. 27:502 (1969).
V. Oyama and H. Eagle, Measurement of cell growth in tissue culture with a phenol reagent (Folin—Ciocalteau), Proc. Soc. Exp. Biol. Med. 91:305 (1956).
T. Yau and J. Mencl, A study of the peroxidation of fatty acid micelles promoted by ionizing radiation, hyrogen peroxide and ascorbate, Int. J. Radiat. Biol. 40:47 (1981).
J. E. Biaglow, M. E. Varnes, E. Clark, E. Epp, The role of thiols in cellular response to radiation and drugs, Radiat. Res. 95: 437 (1983).
M. E. Varnes, S. Tuttle, J. E. Biaglow, Nitroheterocycle metabolism in mammalian cells: Stimulation of the hexose monophosphate shunt, Biochem. Pharmacol. 33:1671 (1984).
L. Flohe and I. Brand, Kinetics of glutathione peroxidase, Biochim. Biophys. Acta. 191:541 (1969).
C. Little, R. Olinescu, K. G. Reid, and P. J. O’Brien, Properties and Regulation of glutathione peroxidase, J. Biol. Chem. 245:3632 (1970).
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© 1986 Plenum Press, New York
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Donahue, L., Biaglow, J.E., Varnes, M.E. (1986). Use of the Oxidase Electrode to Determine Tumor Cell Metabolism of Peroxide. In: Longmuir, I.S. (eds) Oxygen Transport to Tissue VIII. Advances in Experimental Medicine and Biology, vol 200. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5188-7_70
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DOI: https://doi.org/10.1007/978-1-4684-5188-7_70
Publisher Name: Springer, Boston, MA
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