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Platelet Membrane Enzymes and Hemostasis

  • Chapter
The Enzymes of Bioligical Membranes

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Abstract

Blood platelets or thrombocytes are small, enucleate but metabolically active cells that are intimately involved in the normal hemostatic responses to vascular injury. They also play a major, if ill-defined, role in the pathology of cardiovascular and cerebrovascular disease. In addition to the widespread problems of coronary thrombosis, stroke, and the like, several bleeding disorders have been identified that are due to defective platelet functions.

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References

  • Abramowitz, J., Malik, M. N., Stracher, A., and Detwiler, T. C., 1972, Studies on the contractile proteins from blood platelets, Cold Spring Harbor Symp. Quant. Biol. 37:595.

    Google Scholar 

  • Abramson, H. A., 1928, The electrophoresis of the blood platelets of the horse with reference to their origin and thrombus formation, J. Exp. Med. 47:677.

    PubMed  CAS  Google Scholar 

  • Adelstein, R. S., and Conti, M. A., 1972, The characterization of contractile proteins from platelets and fibroblasts, Cold Spring Harbor Symp. Quant. Biol. 37:599.

    Google Scholar 

  • Adelstein, R. S., Pollard, T. D., and Kuehl, W. M., 1971, Isolation and characterization of myosin and two myosin fragments from human blood platelets, Proc. Natl. Acad. Sci. U.S.A. 68:2703.

    PubMed  CAS  Google Scholar 

  • Adelstein, R. S., Conti, M. A., and Anderson, W., 1973, Phosphorylation of human platelet myosin, Proc. Natl. Acad. Sci. U.S.A. 70:3115.

    PubMed  CAS  Google Scholar 

  • Ardlie, N. G., 1968; ADP-induced platelet aggregation without a plasma cofactor, Fed. Proc. Fed. Am. Soc. Exp. Biol 27:321.

    Google Scholar 

  • Baenziger, N. L., Brodie, G. N., and Majerus, P. W., 1971, A thrombin-sensitive protein of human platelet membranes, Proc. Natl. Acad. Sci. U.S.A. 68:240.

    PubMed  CAS  Google Scholar 

  • Bangham, A. D., Pethiga, B. A., and Seaman, G. V. F., 1958, The charged groups at the interface of some blood cells, Biochem. J. 69:12.

    PubMed  CAS  Google Scholar 

  • Barber, A. J., and Jamieson, G. A., 1970, Isolation and characterization of plasma membranes from human blood platelets, J. Biol. Chem. 245:6357.

    PubMed  CAS  Google Scholar 

  • Barber, A. J., and Jamieson, G. A., 1971a, Platelet collagen adhesion: Characterization of collagen glucosyltransferase of plasma membranes, Biochim. Biophys. Acta 252:533.

    PubMed  CAS  Google Scholar 

  • Barber, A. J., and Jamieson, G. A., 1971b, Characterization of membrane-bound collagen galactosyl-transferase of human blood platelets, Biochim. Biophys. Acta 252:546.

    PubMed  CAS  Google Scholar 

  • Barber, A. J., Pepper, D. S., and Jamieson, G. A., 1971, A comparison of methods for platelet lysis and the isolation of platelet membranes, Thromb. Diath. Haemorrh. 26:38.

    PubMed  CAS  Google Scholar 

  • Barton, P. G., 1967, Sequence theories of blood coagulation re-evaluated with reference to lipid-protein interactions, Nature (London) 215:1508.

    CAS  Google Scholar 

  • Barton, P. G., 1968, The effect of surface change density of phosphatides on the binding of cations, J. Biol. Chem. 243:3884.

    PubMed  CAS  Google Scholar 

  • Barton, P. G., 1969, Lipoproteins of blood coagulation, in: Structural and Functional Aspects of Lipoproteins in Living Systems (A. Scanu and E. Tria, eds.), pp. 465–515, Academic Press, London.

    Google Scholar 

  • Barton, P. G., 1971, Molecular mechanisms of prothrombin activation, Proc. 2nd Congr. Int. Soc. Thromb. Haemostasis, Oslo, p. 4.

    Google Scholar 

  • Barton, P. G., and Hanahan, D. J., 1969, Some lipid-protein interactions involved in prothrombin activation, Biochim. Biophys. Acta 187:319.

    PubMed  CAS  Google Scholar 

  • Barton, P. G., and Jevons, S., 1970, Interactions of phosphatides with some paraffin chain salts, Chem. Phys. Lipids 4:289.

    CAS  Google Scholar 

  • Barton, P. G., Yin, E. T., and Wessler, S., 1970, Reactions of factor X-phosphatide mixtures in vitro and in vivo, J. Lipid Res. 11:87.

    PubMed  CAS  Google Scholar 

  • Baumgartner, H. R., and Born, G. V. R., 1968, Effects of 5-hydroxytryptamine on platelet aggregation, Nature (London) 218:137.

    CAS  Google Scholar 

  • Becker, G. A., Chalos, M. K., Tucgelli, M., and Aster, R. H., 1972, Prostaglandin E1 in preparation and storage of platelet concentrates, Science 175:538.

    PubMed  CAS  Google Scholar 

  • Bender, N., Fasold, H., and Rack, M., 1974, Interaction of rabbit muscle actin and chemically modified actin with ATP, ADP and protein reactive analogues; Role of the nucleotide, FEBS Lett. 44:209.

    PubMed  CAS  Google Scholar 

  • Benson, B. J., Kisiel, W., and Hanahan, D. J., 1973, Calcium binding and other characteristics of bovine factor II and its activation intermediates, Biochim. Biophys. Acta 329:81.

    PubMed  CAS  Google Scholar 

  • Bettex-Galland, M., and Lüscher, E. F., 1959, Extraction of an actomyosin-like protein from human thrombocytes, Nature (London) 184:276.

    CAS  Google Scholar 

  • Bettex-Galland, M., and Lüscher, E. F., 1965, Thrombosthenin, the contractile protein from blood platelets and its relation to other contractile proteins, Adv. Prot. Res. 20:1.

    CAS  Google Scholar 

  • Bettex-Galland, M., Portzehl, H., and Lüscher, E. F., 1963, Dissociation of thrombosthenin into two components. Investigation of ATPase activity, Helv. Chim. Acta 46:1595.

    CAS  Google Scholar 

  • Bettex-Galland, M., Lüscher, E. F., and Weibel, E. R., 1969, Thrombosthenin—electron microscopical studies on its localization in human blood platelets and some properties of its subunits, Thromb. Diath. Haemorrh. 22:431.

    PubMed  CAS  Google Scholar 

  • Booyse, F. M., Hoveke, T. P., Zschocke, D., and Rafelson, M. E., 1971a, Human platelet myosin; Isolation and properties, J. Biol. Chem. 246:4291.

    PubMed  CAS  Google Scholar 

  • Booyse, F. M., Sternberger, L. A., Zschocke, D., and Rafelson, M. E. Jr., 1971b, Ultrastructural localization of a contractile (thrombosthenin) system in human platelets using a labelled antibodyperoxidase staining technique, J. Histochem. Cytochem. 19:540.

    PubMed  CAS  Google Scholar 

  • Booyse, F. M., Hoveke, T. P., and Rafelson, M. E., 1973a, Human platelet actin: Isolation and properties, J. Biol. Chem. 248:4083.

    PubMed  CAS  Google Scholar 

  • Booyse, F. M., Giuliani, D., Marr, J. J., and Rafelson, M. E., 1973b, Cyclic adenosine 3′,5′-monophosphate dependent protein kinase of human platelets: Membrane phosphorylation and regulation of platelet function, Ser. Haematol. 6:351.

    PubMed  CAS  Google Scholar 

  • Born, G. V. R., 1962, Aggregation of blood platelets by adenosine diphosphate and its reversal, Nature (London) 194:927.

    CAS  Google Scholar 

  • Born, G. V. R., 1964, Strong inhibition by 2-chloroadenosine of the aggregation of blood platelets by adenosine diphosphate, Nature (London) 202:95.

    CAS  Google Scholar 

  • Born, G. V. R., 1965, Uptake of adenosine and adenosine diphosphate by human blood platelets, Nature (London) 206:1121.

    CAS  Google Scholar 

  • Bosmann, H. G., 1970, Glycoprotein biosynthesis: Purification and properties of glycoprotein N-acetylglucosaminyl transferases from guinea pig liver utilizing endogenous and exogenous acceptors, Eur. J. Biochem. 14:33.

    PubMed  CAS  Google Scholar 

  • Boullin, D. J., Green, A. R., and Price, K. S., 1972, The mechanism of adenosine diphosphate induced platelet aggregation: Binding to platelet receptors and inhibition of binding and aggregation by prostaglandin E 1, J. Physiol. 221:415.

    PubMed  CAS  Google Scholar 

  • Brodie, G. N., Baenziger, N. L., Chase, L. R., and Majerus, P. W., 1972, The effects of thrombin on adenyl cyclase activity and a membrane protein from human platelets, J. Clin Invest. 51:81.

    PubMed  CAS  Google Scholar 

  • Bull, R. K., Jevons, S., and Barton, P. G., 1972, Complexes of prothrombin with calcium ions and phospholipids, J. Biol. Chem. 247:2747.

    PubMed  CAS  Google Scholar 

  • Bungenberg de Jong, H. G., 1949, Reversal of charge phenomena, equivalent weight and specific properties of ionized groups, in: Colloid Science, Vol. 2 (H. R. Kruyt, ed.), Chapter IX, Elsevier, Amsterdam.

    Google Scholar 

  • Castaldi, P. A., Firkin, B. G., Blagkwell, P. M., and Clifford, K. I., 1962, An electron microscopic study of the changes in platelets during viscous metamorphosis, Blood 20:566.

    PubMed  CAS  Google Scholar 

  • Castaldi, P. A., Larrieu, M. J., and Caen, J., 1965, Availability of platelet factor 3 and activation of factor XII in thrombasthenia, Nature (London) 207:422.

    CAS  Google Scholar 

  • Cazenave, J.-P., Guccione, M. A., Mustard, J. F., and Pagkham, M. A., 1974a, Lack of effect of UDP, UDPG, and glucosamine on platelet reactions with collagen, Thromb. Diath. Haemorrh. 31:521.

    PubMed  CAS  Google Scholar 

  • Cazenave, J.-P., Pagkham, M. A., and Mustard, J. F., 1974b, Adherence of platelets to a collagen-coated surface: development of a quantitative method, J. Lab. Clin. Med. 82:978.

    Google Scholar 

  • Chambers, D. A., Salzman, E. W., and Neri, L. L., 1967, Characterization of “Ecto-ATPase” of human platelets, Arch. Biochem. Biophys. 119:173.

    PubMed  CAS  Google Scholar 

  • Chap, H., and Douste-Blazy, L., 1974, Phospholipase C-induced release reaction in platelets, Eur. J. Biochem. 48:351.

    PubMed  CAS  Google Scholar 

  • Chesney, C., Harper, E., and Colman, R. W., 1972, Critical role of the carbohydrate side chain of collagen in platelet aggregation, Fed. Proc. Fed. Am. Soc. Exp. Biol. 31:242 (abstract).

    Google Scholar 

  • Chuang, T. F., Sargeant, R. B., and Hougie, C., 1972, The intrinsic activation of factor X in blood coagulation, Biochim. Biophys. Acta 273:287.

    PubMed  CAS  Google Scholar 

  • Chuang, T. F., Sargeant, R. B., and Hougie, C., 1974, The effect of calcium ions on the properties of factor IX and its activated form, Br. J. Haematol. 27:281.

    PubMed  CAS  Google Scholar 

  • Clayton, S., and Cross, M. J., 1963, The aggregation of blood platelets by catecholamines and by thrombin, J. Physiol. 169:82P.

    Google Scholar 

  • Cohen, I., and Cohen, C., 1972, A tropomyosin-like protein from human platelets, J. Mol. Biol. 68:383.

    PubMed  CAS  Google Scholar 

  • Cohen, I., Bohak, Z., DeVries, A., and Katghalsky, E., 1969, Thrombosthenin M: Purification and interaction with thrombin, Eur. J. Biochem. 10:388.

    PubMed  CAS  Google Scholar 

  • Cohen, P., Broekman, M. J., Verkley, A., Lisman, J. W. W., and Derksen, A., 1971, Quantification of human platelet inositides and the influence of ionic environment on their incorporation of orthophosphate-32P, J. Clin. Invest. 50:762.

    PubMed  CAS  Google Scholar 

  • Cole, E. R., Koppel, J. L., and Olwin, J. H., 1964, Interaction of bovine autoprothrombin C with phospholipids and divalent cations, Can. J. Biochem. 42:1595.

    PubMed  CAS  Google Scholar 

  • Cole, E. R., Koppel, J. L., and Olwin, J. H., 1965, Phospholipid-protein interactions in the formation of prothrombin activator, Thromb. Diath. Haemorrh. 14:431.

    PubMed  CAS  Google Scholar 

  • Davey, M. G., and Lüscher, E. F., 1967, Actions of thrombin and other coagulant and proteolytic enzymes on blood platelets, Nature (London) 216:857.

    CAS  Google Scholar 

  • Deppert, W., Wenchau, H., Walter, G., 1974, Differentiation between intracellular and cell surface glycosyl transferases: Galactosyl transferase activity in intact cells and in cell homogenate, Proc. Natl. Acad. Sci. U.S.A. 71:3068.

    PubMed  CAS  Google Scholar 

  • Deykin, D., and Dresser, R. K., 1968, The incorporation of acetate and palmitate into lipids by human platelets, J. Clin. Invest. 47:1590.

    PubMed  CAS  Google Scholar 

  • Eika, C and Abilgaard, U., 1970, Inhibition of thrombin-induced aggregation of human platelets by antithrombin III, Scand. J. Haematol. 7:460.

    PubMed  CAS  Google Scholar 

  • Elgjo, R. F., and Hovig, T., 1972, Ultrastructural studies of platelet “activation” and aggregation in normal spleen from different species, Scand. J. Haematol. 9:587.

    PubMed  CAS  Google Scholar 

  • Elgsaeter, A., and Branton, D., 1974, Intramembrane particle aggregation in erythrocyte ghosts. I. The effect of protein removal, J. Cell Biol. 63:1018.

    PubMed  CAS  Google Scholar 

  • Esnouf, M. P., and Jobin, F., 1965, Lipids in prothrombin conversion, Thromb. Diath. Haemorrh. Suppl. 17:103.

    PubMed  CAS  Google Scholar 

  • Feinman, R. D., and Detwiler, T. C., 1974, Platelet secretion induced by divalent cation ionophores, Nature (London) 249:172.

    CAS  Google Scholar 

  • Fisher, D. B., and Mueller, G. C., 1968, Turnover of phosphatidyl inositol after phytohaemagglutinin stimulation of T-cells, Proc. Natl. Acad. Sci. U.S.A. 60:1396.

    PubMed  CAS  Google Scholar 

  • Gaarder, A., and Laland, S., 1964, Hypothesis for the aggregation of platelets by nucleotides, Nature (London) 202:909.

    CAS  Google Scholar 

  • Gaarder, A., Jonsen, J., Laland, S., Hellem, A. J., and Owren, P. A., 1961, Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets, Nature (London) 192:531.

    CAS  Google Scholar 

  • Ganguly, P., 1974, Binding of thrombin to human platelets, Nature (London) 247:306.

    CAS  Google Scholar 

  • Girardot, J.-M., Delaney, R., and Connor Johnson, B., 1974, Carboxylation, the completion step in prothrombin biosynthesis, Biochim. Biophys. Acta 59:1197.

    CAS  Google Scholar 

  • Gitel, S. N., Owen, W. G., Esmon, C. T., and Jackson, C. M., 1973, A polypeptide region of bovine prothrombin specific for binding to phospholipids, Proc. Natl. Acad. Sci. U.S.A. 7:1344.

    Google Scholar 

  • Gold, M., Evensen, S. A., Belamarich, F. A., and Shepro, D., 1973, Platelet factor 3 made available from human platelets by ADP: Inhibition by colchicine, Thromb. Diath. Haemorrh. 30:155.

    PubMed  CAS  Google Scholar 

  • Gough, G., Maguire, M. H., and Penglis, F., 1972, Analogues of adenosine-5′-diphosphate-new platelet aggregators: Influence of purine ring and phosphate chain substitutions on the platelet-aggregating potency of adenosine-5′-diphosphate, Mol. Pharmacol. 8:170.

    PubMed  CAS  Google Scholar 

  • Gross, R. W., Schneider, W., Kaulen, D. H., and Reuter, H., 1972, Platelet metabolism with special reference to compartments and membranes, Ann. N. Y. Acad. Sci. 201:84.

    CAS  Google Scholar 

  • Guccione, M. A., Packham, M. A., Kinlough-Rathbone, R. L., and Mustard, J. F., 1971, Reactions of 14C-ADP and 14C-ATP with washed platelets from rabbits, Blood 37:542.

    PubMed  CAS  Google Scholar 

  • Hamberg, M., and Samuelsson, B., 1974, Prostaglandin endoperoxides. Novel transformations of arachi-donic acid in human platelets, Proc. Natl. Acad. Sci. U.S.A. 71:3400.

    PubMed  CAS  Google Scholar 

  • Hamberg, M., Svensson, J., Wakabayashi, T., and Samuelsson, B., 1974, Isolation and structure of two prostaglandin endoperoxides that cause platelet aggregation, Proc. Natl. Acad. Sci. U.S.A. 71:345.

    PubMed  CAS  Google Scholar 

  • Hampton, J. R., and Mitchell, J. R. A., 1966, Modification of the platelet electrokinetic response to aggregating agents, Nature (London) 210:1000.

    CAS  Google Scholar 

  • Hampton, J. R., and Mitchell, J. R. A., 1974, Platelet electrophoresis: The present position, Thromb. Diath. Haemorrh. 31:204.

    PubMed  CAS  Google Scholar 

  • Hanahan, D. J., and Papahadjopoulos, D., 1965, Interactions of phospholipids with coagulation factors, Thromb. Diath. Haemorrh. Suppl. 17:72.

    Google Scholar 

  • Han, P., and Ardlie, N. G., 1974, Platelet aggregation and release by ADP and thrombin: Evidence for two separate effects of ADP on platelets, involvement of fibrinogen in release, and mechanism of inhibitory action of acetylsalicyclic acid, Br. J. Haematol. 26:357.

    PubMed  CAS  Google Scholar 

  • Hanson, J., Repke, D., Katz, A. M., and Aledort, L. M., 1973, Calcium ion control of platelet thrombosthenin ATPase activity, Biochim. Biophys. Acta 314:382.

    PubMed  CAS  Google Scholar 

  • Hardisty, R. M., and Hutton, R. A., 1966, Platelet aggregation and availability of platelet factor 3, Br. J. Haematol. 12:764.

    PubMed  CAS  Google Scholar 

  • Hardisty, R. M., and Hutton, R. A., 1967, Bleeding tendency associated with a new abnormality of platelet behaviour, Lancet 1:983.

    PubMed  CAS  Google Scholar 

  • Hardisty, R. M., Hutton, R. A., Montgomery, D., Richard, S., and Trebilcock, 1970, Secondary platelet aggregation: A quantitative study, Br. J. Haematol. 19:307.

    PubMed  CAS  Google Scholar 

  • Harris, G. L. A., and Crawford, N., 1973, Isolation of pig platelet membranes and granules. Distribution and validity of marker enzymes, Biochim. Biophys. Acta 291:701.

    PubMed  CAS  Google Scholar 

  • Haslam, R. J., 1964, Role of adenosine diphosphate in the aggregation of human blood platelets by thrombin and by fatty acids, Nature (London) 202:765.

    CAS  Google Scholar 

  • Haslam, R. J., 1967, Mechanisms of blood platelet aggregation, in: Physiology of Hemostasis and Thrombosis (S. A. Johnson and W. H. Seegers, eds.), pp. 88–112, Charles C Thomas, Springfield, Illinois.

    Google Scholar 

  • Haslam, R. J., and Lynham, J. A., 1973, Activation and inhibition of blood platelet adenylate cyclase by adenosine or 2-chloroadenosine, Life Sci. 11:1143.

    Google Scholar 

  • Heldebrant, C. M., and Mann, K. G., 1973, The activation of prothrombin I. Isolation and preliminary characterization of intermediates, J. Biol. Chem. 248:3642

    PubMed  CAS  Google Scholar 

  • Hellem, A. J., 1960, The adhesiveness of human blood platelets in vitro, Scand. J. Clin. Invest. 12(Suppl. 51):1.

    PubMed  Google Scholar 

  • Hemker, H. C., and Kahn, M. J. P., 1967, Reaction sequence of blood coagulation, Nature (London) 215:1201.

    CAS  Google Scholar 

  • Hemker, H. C., Esnouf, M. P., Hemker, P. W., Swart, A. C. W., and MacFarlane, R. G., 1967, Formation of prothrombin converting activity, Nature (London) 215:248.

    CAS  Google Scholar 

  • Hemker, H. C., Kahn, M.J. P., and Devilee, P. P., 1970, The adsorption of coagulation factors onto phospholipids: Its role in the reaction mechanism of blood coagulation, Thromb. Diath. Haemorrh. 24:214.

    PubMed  CAS  Google Scholar 

  • Heppel, L. A., 1971, The concept of periplasmic enzymes, in: Structure and Function of Biological Membranes (L. I. Rothfield, ed.), pp. 223–247, Academic Press, New York.

    Google Scholar 

  • Hoak, J. C., 1972, Freeze-etching studies of human platelets, Blood 40:514.

    PubMed  CAS  Google Scholar 

  • Holmsen, H., Day, H. J., and Setkowsky, C. A.4, 1972, Secretory mechanisms: Behaviour of adenine nucleotides during the platelet release reaction induced by adenosine diphosphate and adrenaline, Biochem. J. 129:67.

    PubMed  CAS  Google Scholar 

  • Horák, H., and Barton, P. G., 1974, Effects of α,β -methylene-adenosine-5′-diphosphate on blood platelet aggregation, Biochim. Biophys. Acta 373:471.

    PubMed  Google Scholar 

  • Horowitz, H. I., and Papayoanou, M. F., 1969, Activation of platelet factor 3 by adenosine 5′-diphosphate, Thromb. Diath. Haemorrh. 19:18.

    Google Scholar 

  • Hougie, C., Denson, K. W. E., and Biggs, R., 1967, A study of the reaction product of factor VIII and factor IX by gel filtration, Thromb. Diath. Haemorrh. 18:211.

    PubMed  CAS  Google Scholar 

  • Hovig, T., 1970, Blood platelet surface and shape. A scanning electron microscopic study, Scand. J. Haematol. 7:420.

    PubMed  CAS  Google Scholar 

  • Hugues, J., and Mahieu, P., 1970, Platelet aggregation induced by basement membranes, Thromb. Diath. Haemorrh. 24:395.

    PubMed  CAS  Google Scholar 

  • Jackson, C. M., Owen, W. G., Gitel, S. N., and Esmon, C. T., 1974, The chemical role of lipids in prothrombin conversion, Thromb. Diath. Haemorrh. Suppl. 57:273.

    CAS  Google Scholar 

  • Jamieson, G. A., 1973, Role of glycoproteins in platelet function, in:Erythrocytes, Thrombocytes and Leukocytes (E. Gerlach, K. Moser, E. Deutsch, and W. Wilmanns, eds.), pp. 209–210, Georg Thieme, Stuttgart.

    Google Scholar 

  • Jamieson, G. A., and Barber, A. J., 1973, Biochemistry of platelet membranes, Thromb. Diath. Haemorrh. Suppl. 54:239.

    Google Scholar 

  • Jamieson, G. A., Urban, C. L., and Barber, A. J., 1971, Enzymatic basis for platelet: collagen adhesion as a primary step in haemostasis, Nature (London) New Biol. 234:5.

    CAS  Google Scholar 

  • Jevons, S., and Barton, P. G., 1971, Biochemistry of blood platelets. Interaction of activated factor X with platelets, Biochemistry 10:428.

    PubMed  CAS  Google Scholar 

  • Jobin, F., and Esnouf, M. P., 1967, Studies on the formation of the prothrombin-converting complex, Biochem. J. 102:666.

    PubMed  CAS  Google Scholar 

  • Joist, J. H., Dolezel, G., Lloyd, J. V., and Mustard, J. F., 1974, Phospholipid transfer between plasma and platelets in vitro Thromb. Res., 4 (suppl. 1):81.

    Google Scholar 

  • Karpatkin, S., 1967, Studies on human platelet glycolysis. Effect of glucose, cyanide, insulin, citrate and agglutination and contraction on platelet glycolysis, J. Clin. Invest. 46:409.

    PubMed  CAS  Google Scholar 

  • Kaulen, H. D., and Gross, R., 1973, Metabolic properties of human platelet membranes. I. Characterization of platelet membranes prepared by sucrose and Ficoll density gradients, Thromb. Diath. Haemorrh. 30:199.

    PubMed  CAS  Google Scholar 

  • Lapetina, E. G., and Mitchell, R. H., 1973, Phosphatidyl inositol metabolism in cells receiving extracellular stimulation, FEBS Lett. 31:1.

    PubMed  CAS  Google Scholar 

  • Legrand, Y., and Pignaud, G., 1971, Some factors influencing the aggregating property of collagen, Acta Medica Scand. Suppl. 525:127.

    CAS  Google Scholar 

  • Lloyd, J. V., and Mustard, J. F., 1974, Changes in 32P-content of phosphatidic acid and phosphatidyl inositol of rabbit platelets during aggregation induced by collagen or thrombin, Br. J. Haematol. 26:243.

    PubMed  CAS  Google Scholar 

  • Lloyd, J. V., Nishizawa, E. E., Haldar, J., and Mustard, J. F., 1972, Changes in 32P-labelling of platelet phospholipids in response to ADP, Br. J. Haematol. 23:571.

    PubMed  CAS  Google Scholar 

  • MacMillan, D. C., 1966, Secondary clumping effect in human citrated platelet-rich plasma produced by adenosine diphosphate and adrenaline, Nature (London) 211:140.

    CAS  Google Scholar 

  • Maeda, T., and Ohnishi, S.-L., 1974, Membrane fusion: Transfer of phospholipid bilayer membranes, Biochem. Biophys. Res. Commun. 60:1509.

    PubMed  CAS  Google Scholar 

  • Magnusson, S., Sottnup-Jensen, L., and Petersen, T. E., 1974, Primary structure of the vitamin K-dependent part of prothrombin, FEBS Lett. 44:189.

    PubMed  CAS  Google Scholar 

  • Maguire, M. H., and Michal, F., 1968, Powerful new aggregator of blood platelets—2-chloro-adenosine-5′-diphosphate, Nature (London) 217:571.

    CAS  Google Scholar 

  • Majerus, P. W., Smith, M. B., and Clamon, G. H., 1969, Lipid metabolism in human platelets. I. Evidence for a complete fatty acid synthesizing system, J. Clin. Invest. 48:156.

    PubMed  CAS  Google Scholar 

  • Malhotra, O. P., 1972, Atypical prothrombins induced by dicoumarol, Nature (London) 239:59.

    CAS  Google Scholar 

  • Malik, M. N., Detwiler, T. C., and Stracher, A., 1973, Allosteric regulation of platelet actomyosin, Biochem. Biophys. Res. Commun. 55:912.

    PubMed  CAS  Google Scholar 

  • Malik, M. N., Rosenberg, S., Detwiler, T. C., and Stracher, A., 1974, Role of Ca2+ ions in the allosteric regulation of platelet actomyosin, Biochem. Biophys. Res. Commun. 61:1071.

    PubMed  CAS  Google Scholar 

  • Mannherz, H. G., Schenck, H., and Goody, R. S., 1974, Synthesis of ATP from ADP and Pi at the myosin-subfragment-1 active site, Eur. J. Biochem. 48:287.

    PubMed  CAS  Google Scholar 

  • Marcus, A. J., 1972, Recent advances in platelet lipid metabolism research, Ann. N.Y. Acad. Sci. 201:102.

    PubMed  CAS  Google Scholar 

  • Marcus, A. J., 1973, Observations on the structure and function of human platelet membranes, in: Erythrocytes, Thrombocytes and Leukocytes (E. Gerlach, K. Moser, E. Deutsch, and W. Wilmanns, eds.), pp. 206–208, Georg Thieme, Stuttgart.

    Google Scholar 

  • Marcus, A. J., Zucker-Franklin, D., Safier, L. B., and Ullman, H. L., 1966, Studies on human platelet granules and membranes, J. Clin. Invest. 45:14.

    PubMed  CAS  Google Scholar 

  • Marcus, A. J., Ullman, H. L., and Safier, L. B., 1969, Lipid composition of subcellular particles of human blood platelets, J. Lipid Res. 10:108.

    PubMed  CAS  Google Scholar 

  • Marcus, A. J., Safier, L. B., and Ullman, H. L., 1971, Functions of platelet membranes, in: The Circulating Platelet (S. A. Johnson, ed.), pp. 241–258, Academic Press, New York.

    Google Scholar 

  • Marston, S., and Lehman, W., 1974, ADP-binding to relaxed scallop myofibrils, Nature (London) 252:38.

    CAS  Google Scholar 

  • McDonald, J. W. D., and Stuart, R. K., 1974, Interaction of prostaglandins E1 and E2 in regulation of cyclic AMP and aggregation in human platelets: evidence for a common prostaglandin receptor, J. Lab. Clin. Med. 84:111.

    PubMed  CAS  Google Scholar 

  • McElroy, R. A., Kinlough-Rathbone, R. L., Ardlie, N. G., Packham, M. A., and Mustard, J. F., 1971, The effect of aggregating agents on oxidative metabolism of rabbit platelets, Biochim. Biophys. Acta 253:64.

    PubMed  CAS  Google Scholar 

  • McLean, J. R., and Veloso, H., 1967, Change of shape without aggregation caused by ADP in rabbit platelets at low pH, Life Sci. 6:1983.

    PubMed  CAS  Google Scholar 

  • Mehrishi, J. N., 1970, Phosphate groups (receptors?) on the surface of human blood platelets, Nature (London) 226:452.

    CAS  Google Scholar 

  • Mehrishi, J. N., and Grassetti, D. R., 1969, Sulfhydryl groups on the surface of intact Ehrlich ascites tumor cells, human blood platelets and lymphocytes, Nature (London) 224:563.

    CAS  Google Scholar 

  • Mills, D. C. B., and MacFarlane, D. E., 1974, Stimulation of human platelet adenylate cyclase by PGE D2, Thromb. Res. 5:401.

    PubMed  CAS  Google Scholar 

  • Mills, D. G. B., and Roberts, G. G. K., 1967a, Membrane-active drugs and the aggregation of human platelets, Nature (London) 193:443.

    CAS  Google Scholar 

  • Mills, D. C. B., and Roberts, G. G. K., 1967b, Effects of adrenaline on human platelets, J. Physiol. 193:443.

    PubMed  CAS  Google Scholar 

  • Mills, D. C. B., and Smith, J. B., 1971, The influence on platelet aggregation of drugs that affect the accumulation of adenosine 3’:5’-cyclic monophosphate in platelets, Biochem. J. 121:185.

    PubMed  CAS  Google Scholar 

  • Mills, D. G. B., Robb, I. A., and Roberts, G. C. K., 1968, The release of nucleotides, 5-hydroxy-tryptamine and enzymes from human blood platelets during aggregation, J. Physiol. 195:715.

    PubMed  CAS  Google Scholar 

  • Mitchell, J. R. A., and Sharp, A. A., 1964, Platelet clumping in vitro, Br. J. Haematol. 10:78.

    PubMed  CAS  Google Scholar 

  • Muggli, R., and Baumgartner, H. R., 1973, Collagen-induced platelet aggregation: Requirement for tropocollagen multimers, Thromb. Res. 3:715.

    CAS  Google Scholar 

  • Mürer, E. H., 1968, Release reaction and energy metabolism in blood platelets with special reference to the burst in oxygen uptake, Biochim. Biophys. Acta 162:320.

    PubMed  Google Scholar 

  • Murphy, M. J., 1972, The shape of blood platelets. An implication of lyophilization and scanning electron microscopy, Thromb. Diath. Haemorrh. 28:237.

    PubMed  Google Scholar 

  • Mustard, J. F., and Packham, M. A., 1970, Factors influencing platelet function: Adhesion, release and aggregation, Pharmacol. Rev. 22:97.

    PubMed  CAS  Google Scholar 

  • Mustard, J. F., Hegardt, B., Rowsell, H. C., and MagMillan, R. L., 1964, Effect of adenosine nucleotides on platelet aggregation and clotting time, J. Lab. Clin. Med. 64:548.

    PubMed  CAS  Google Scholar 

  • Muszbek, L., and Laki, K., 1974, Cleavage of actin by thrombin, Proc. Natl. Acad. Sci. U.S.A. 71:2208.

    PubMed  CAS  Google Scholar 

  • Naghman, R. L., and Ferris, B., 1970, Human platelet membrane protein, Biochemistry 9:200.

    Google Scholar 

  • Nachman, R. L., and Ferris, B., 1972, Studies on the proteins of human platelet membranes, J. Biol. Chem. 247:4468.

    PubMed  CAS  Google Scholar 

  • Nachman, R. L., and Ferris, B., 1973, Iodination of the human platelet membrane. Studies on the major surface glycoprotein, J. Biol. Chem. 248:2928.

    PubMed  CAS  Google Scholar 

  • Nachman, R. L., and Ferris, B., 1974, Binding of adenosine diphosphate by isolated membranes from human platelets, J. Biol. Chem. 249:704.

    PubMed  CAS  Google Scholar 

  • Nachman, R. L., Marcus, A. J., and Zucker-Franklin, D., 1967a, Immunologic studies of proteins associated with subcellular fractions of normal human platelets, J. Lab. Clin. Med. 69:651.

    PubMed  CAS  Google Scholar 

  • Nachman, R. L., Marcus, A. J., and Safier, L. B., 1967b, Platelet thrombosthenin: Subcellular localization and function, J. Clin. Invest. 46:1380.

    PubMed  CAS  Google Scholar 

  • Nakao, K., and Angrist, A. A., 1968, Membrane surface specialization of blood platelet and megakaryocyte, Nature (London) 217:960.

    CAS  Google Scholar 

  • Nelsestuen, G. L., and Suttie, J. W., 1972, Mode of action of vitamin K. Calcium binding properties of bovine prothrombin, Biochemistry 11:4961.

    PubMed  CAS  Google Scholar 

  • Nelsestuen, G. L., and Zytkovicz, T. H., 1974, The mode of action of vitamin K. Identification of γ-carboxyglutamic acid as a component of prothrombin, J. Biol. Chem. 249:6347.

    PubMed  CAS  Google Scholar 

  • Nishizawa, E. E., Hovig, T., Lotz, F., Rowsell, H. C., and Mustard, J. F., 1969, Effect of a natural phosphatidyl serine fraction on blood coagulation, platelet aggregation and haemostasis, Br. J. Haematol. 16:487.

    PubMed  CAS  Google Scholar 

  • O’Brien, J. R., 1963, Some effects of adrenaline and anti-adrenaline on platelets in vitro and in vivo, Nature (London) 200:763.

    Google Scholar 

  • Øllgaard, E., 1961, Macroscopic studies of platelet aggregation. Nature of an aggregating factor in red blood cells and platelets, Thromb. Diath. Haemorrh. 6:86.

    PubMed  Google Scholar 

  • Owen, W. G., Esmon, C. T., and Jackson, C. M., 1974, The conversion of prothrombin to thrombin. I. Characterization of the reaction products formed during the activation of bovine prothrombin, J. Biol. Chem. 249:594.

    PubMed  CAS  Google Scholar 

  • Oxender, D. L., 1972, Membrane Transport, Ann. Rev. Biochem. 41:777.

    PubMed  CAS  Google Scholar 

  • Papahadjopoulos, D., and Hanahan, D. J., 1964, Observations on the interactions of phospholipids and certain clotting factors in prothrombin activator formation, Biochim. Biophys. Acta 90:436.

    PubMed  CAS  Google Scholar 

  • Papahadjopoulos, D., Mayhew, E., Poste, G., Smith, S., and Vail, W. J., 1974, Incorporation of lipid vesicles by mammalian cells provides a potential method for modifying cell behaviour, Nature (London) 252:163.

    CAS  Google Scholar 

  • Pepper, D. S., and Jamieson, G. A., 1968, Isolation of a glycoprotein fraction from human platelet membranes which inhibits viral haemagglutination, Nature (London) 219:1252.

    CAS  Google Scholar 

  • Pepper, D. S., and Jamieson, G. A., 1969, Studies on glycoproteins. III. Isolation of sialylglycopeptides from human platelet membranes, Biochemistry 8:3362.

    PubMed  CAS  Google Scholar 

  • Pepper, D. S., and Jamieson, G. A., 1970, Isolation of a macroglycopeptide from human platelets, Biochemistry 9:3706.

    PubMed  CAS  Google Scholar 

  • Philip, G., Moran, J., and Colman, R. W., 1970, Dissociation and association of the oligomeric forms of factor V, Biochemistry 9:2212.

    PubMed  CAS  Google Scholar 

  • Phillips, D. R., 1972, Effect of trypsin on the exposed polypeptides and glycoproteins in the human platelet membrane, Biochemistry 11:4582.

    PubMed  CAS  Google Scholar 

  • Phillips, D. R., and Agin, P. P., 1974a, Thrombin substrates and the proteolytic site of thrombin action on human platelet membranes, Biochim. Biophys. Acta 352:218.

    PubMed  CAS  Google Scholar 

  • Phillips, D. R., and Agin, P. P., 1974b, Thrombin interaction with human platelets. Potentiation of thrombin-induced aggregation and release by inactivated thrombin, Thromb. Diath. Haemorrh. 32:207.

    PubMed  CAS  Google Scholar 

  • Probst, E., and Lüscher, E. F., 1972, Studies on thrombosthenin A, the actin-like moiety of the contractile protein from blood platelets. I. Isolation, characterization and evidence for two forms of thrombosthenin A., Biochim. Biophys. Acta 278:577.

    PubMed  CAS  Google Scholar 

  • Puett, D., Wasserman, B. K., Ford, J. D., and Cunningham, L. W., 1973, Collagen-mediated platelet aggregation. Effects of collagen modification involving the protein and carbohydrate moieties, J. Clin. Invest. 52:2495.

    PubMed  CAS  Google Scholar 

  • Puszkin, S., Puszkin, E., Katz, A. M., and Aledort, L. M., 1974, Control of platelet actomyosin activity: Effect of ADP on superprecipitation and ATPase activity of human platelet actomyosin, Biochim. Biophys. Acta 347:102.

    PubMed  CAS  Google Scholar 

  • Radcliffe, R. D., and Barton, P. G., 1972, The purification and properties of activated factor X: Bovine factor X activated with Russell’s viper venom, J. Biol. Chem. 247:7735.

    PubMed  CAS  Google Scholar 

  • Rodman, N. F., 1967a, Platelet ultrastructure, Thromb. Diath. Haemorrh. Suppl. 26:9.

    PubMed  CAS  Google Scholar 

  • Rodman, N. F., 1967b, Sequential changes in platelet ultrastructure in a white thrombus model, in: Physiology of Hemostasis and Thrombosis (S. A. Johnson and W. H. Seegers, eds.), pp. 266–287, Charles C.Thomas, Springfield, Illinois.

    Google Scholar 

  • Roseman, S., 1970, The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion, Chem. Phys. Lipids 5:270.

    PubMed  CAS  Google Scholar 

  • Salzman, E. W., and Neri, L. L., 1969, Cyclic 3′,5 -adenosine monophosphate in human blood platelets, Nature (London) 224:609.

    CAS  Google Scholar 

  • Salzman, E. W., Chambers, D. A., and Neri, L. L., 1966, Possible mechanism of aggregation of blood platelets by adenosine diphosphate, Nature (London) 210:167.

    CAS  Google Scholar 

  • Schmukler, M., and Zuve, P. D., 1972, Studies of glycosidases in fresh and stored human platelets, J. Lab. Clin. Med. 80:635.

    PubMed  CAS  Google Scholar 

  • Seaman, G. V. F., 1967, Surface potential and platelet aggregation, Thromb. Diath. Haemorrh. Suppl. 26:53.

    PubMed  CAS  Google Scholar 

  • Seaman, G. V. F., and Vassar, P. S., 1966, Changes in the electrokinetic properties of platelets during their aggregation, Arch. Biochem. Biophys. 117:10.

    PubMed  CAS  Google Scholar 

  • Shio, H., and Ramwell, P. W., 1972, Prostaglandin E1 in platelet harvesting: An in vitro study, Science 175:536.

    PubMed  CAS  Google Scholar 

  • Siegel, A., Burri, P. H., Weibel, E. R., Bettex-Galland, M., and Lüscher, E. F., 1971, Density gradient centrifugation and electron microscopic characterization of subcellular fractions from human blood platelets, Thromb. Diath. Haemorrh. 25:252.

    PubMed  CAS  Google Scholar 

  • Siraganian, R. P., 1972, Platelet requirement in the interaction of the complement and clotting systems, Nature (London) New Biol. 239:208.

    CAS  Google Scholar 

  • Skoza, L., Zucker, M. B., Jerushalmy, Z., and Grant, R., 1967, Kinetic studies of platelet aggregation induced by adenosine diphosphate and its inhibition by chelating agents, guanidino compounds and adenosine, Thromb. Diath. Haemorrh. 18:713.

    PubMed  CAS  Google Scholar 

  • Smith, J. B., Silver, M. J., Ingerman, C. M., and Kocsis, J. J., 1974, PGE D2 inhibits aggregation of human platelets, Thromb. Res. 5:291.

    PubMed  CAS  Google Scholar 

  • Spaet, T. H., and Cintron, J., 1965, Studies on platelet factor 3 availability, Br. J. Haematol. 11:296.

    Google Scholar 

  • Spaet, T. H., and Lejnieks, I., 1966, Studies on the mechanism whereby platelets are clumped by adenosine diphosphate, Thromb. Diath. Haemorrh. 15:36.

    PubMed  CAS  Google Scholar 

  • Spaet, T. H., and Stemmerman, M. B., 1972, Platelet adhesion, Ann. N.Y. Acad. Sci. 201:13.

    PubMed  CAS  Google Scholar 

  • Spector, A. A., Hoak, J. C., Warner, E. D., and Fry, G. L., 1970, Utilization of long chain free fatty acids by human platelets, J. Clin. Invest. 49:1489.

    PubMed  CAS  Google Scholar 

  • Steiner, M., 1972, Lipid and amino acid synthesis in aggregating platelets, Ann. N.Y. Acad. Sci. 201:92.

    PubMed  CAS  Google Scholar 

  • Steiner, M., 1973, Effect of thrombin on the platelet membrane, Biochim. Biophys. Acta 233:653.

    Google Scholar 

  • Steiner, M., and Kuramoto, A., 1971, Energy metabolism of aggregating platelets. Ser. Haematol. 4:98.

    PubMed  CAS  Google Scholar 

  • Steiner, M., Ando, Y., and Lowenstein, S. R., 1973, Quantification of sulfhydryl and disulfide groups in platelet membranes. Effect of mercaptide formation on aggregation in: Erythrocytes, Thrombocytes, and Leukocytes (E. Gerlach, K. Moser, E. Deutsch, and W. Willmanns, eds.), pp. 206–208, Georg Thieme, Stuttgart.

    Google Scholar 

  • Stenflo, J., and Ganrot, P. O., 1973, Binding of Ca2+ to normal and dicoumarol-induced prothrombin, Biochem. Biophys. Res. Commun. 50:98.

    PubMed  CAS  Google Scholar 

  • Stenflo, J., Fernlund, P., Egan, W., and Roepstorff, P., 1974, Vitamin K dependent modifications of glutamic acid residues in prothrombin, Proc. Natl. Acad. Sci. U.S.A. 71:2730.

    PubMed  CAS  Google Scholar 

  • Sterzing, P. R., and Barton, P. G., 1973, The influence of cholesterol on the activity of phospholipids in blood coagulation: Requirement for a liquid-crystalline lipid phase, Chem. Phys. Lipids 10:137.

    PubMed  CAS  Google Scholar 

  • Stone, J. V., Horák, H., Singh, R. K., and Barton, P. G., 1976, Sulfhydryl analogues of adenosine diphosphate: chemical synthesis and activity as platelet aggregating agents, Can. J. Biochem. 54:540.

    Google Scholar 

  • Tangen, O., McKinnon, E. L., and Berman, H. J., 1973, On the fine structure and aggregation requirements of gel filtered platelets, Scand. J. Haematol. 10:96.

    PubMed  CAS  Google Scholar 

  • Tanner, M. J. A., Boxer, D. H., Gumming, J., and Verrier-Jones, J., 1974, A set of surface proteins common to the circulating human platelet and lymphocyte, Biochem. J. 141:909.

    PubMed  CAS  Google Scholar 

  • Tollefsen, D. M., Feagler, J. R., and Majerus, P. W., 1974, The binding of thrombin to the surface of human platelets, J. Biol. Chem. 249:2646.

    PubMed  CAS  Google Scholar 

  • Valeri, G. R., Zaroulis, C. G., Rogers, J. C., Handin, R. I., and Marghionni, L. D., 1972, Prostaglandins in the preparation of blood components, Science 175:539.

    PubMed  CAS  Google Scholar 

  • Van der Plas, P. M., Van Es, G., Kraan, L., and Hemker, H. G., 1973, The influence of the polyene antibiotic filipin on the extrinsic pathway of blood coagulation, Haemostasis 1:191.

    Google Scholar 

  • Van der Plas, P. M., Kraan, L., Van Es, G., Stibbe, J., and Hemker, H. C., 1974, Effect of the polyene antibiotic filipin on the lipid-dependent intrinsic pathway of blood coagulation, Haemostasis 3:1.

    PubMed  Google Scholar 

  • Vroman, L., 1965, Effects of hydrophobic surfaces upon blood coagulation, Thromb. Diath. Haemorrh. 10:455.

    Google Scholar 

  • Walsh, P. N., 1972, Albumin density gradient separation and washing of platelets and the study of platelet coagulant activities, Br. J. Haematol. 22:205.

    PubMed  CAS  Google Scholar 

  • White, J. G., 1971, Platelet morphology, in: The Circulating Platelet (S. A. Johnson, ed.), pp. 45–121, Academic Press, New York.

    Google Scholar 

  • White, J. G., 1972, Interactions of membrane systems in blood platelets, Am. J. Pathol. 66:295.

    PubMed  CAS  Google Scholar 

  • White, J. G., 1974, Shape change, Thromb. Diath. Haemorrh. Suppl. 60:156.

    Google Scholar 

  • Willingham, M. G., Ostlund, R. E., and Pastan, I., 1974, Myosin is a component of the cell surface of cultured cells, Proc. Natl. Acad. Sci. U.S.A. 71:4144.

    PubMed  CAS  Google Scholar 

  • Wilner, G. D., Nossel, H. L., and LeRoy, E. C., 1968, Aggregation of platelets by collagen, J. Clin. Invest. 47:2616.

    PubMed  CAS  Google Scholar 

  • Zucker, M. B., and Peterson, J., 1967, Serotonin, platelet factor 3 and platelet aggregating agent released by adenosine diphosphate, Blood 30:556.

    Google Scholar 

  • Zucker, M. B., and Peterson, J., 1968, Inhibition of adenosine diphosphate-induced secondary aggregation and other platelet functions by acetylsalicyclic acid ingestion, Proc. Soc. Exp. Biol. Med. 127:547.

    PubMed  CAS  Google Scholar 

  • Zucker-Franklin, D., and Grusky, G., 1972, The actin and myosin filaments of human and bovine blood platelets, J. Clin. Invest. 51:419.

    PubMed  CAS  Google Scholar 

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  1. Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2E1, Canada

    Peter G. Barton

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  1. Department of Biochemistry, School of Medicine, Saint Louis University, USA

    Anthony Martonosi

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Barton, P.G. (1976). Platelet Membrane Enzymes and Hemostasis. In: Martonosi, A. (eds) The Enzymes of Bioligical Membranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2655-7_17

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