Abstract
For research and commercial purposes, the production of materials from cultured cells is progressively becoming routine. Measuring products in cell culture medium, however, is not always straightforward even when the most appropriate product assays are utilized.
To whom correspondence should be addressed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Abbreviations
- PA:
-
plasminogen activator
- HEK:
-
human embryonic kidney
- WHO:
-
World Health Organization
- CTA:
-
Committee on Thrombolytic Agents
- HMW:
-
high molecular weight
- LMW:
-
low molecular weight
- CFES:
-
continuous flow electrophoresis system
References
J.M. Price G.H. Barlow and J.E. Donahoe Mammalian cell culture as a source of biological materials. In “Principles and Techniques of Human Research and Therapeutics A Series of Mographs” F. Gilbert McMahon ed. Futura Publishing company Inc. Mt. Kisko NY XV, 37–54 1978
R. Davis, Technical issues of recombinant DNA in medical products. Presented at Symposium on Medicinals, Chemicals, Diagnostic Products, and Evaluation of New Biotechnology, Stanford Res. Inst., Palo Alto, CA, 1980.
M.B. Bernik and H.C. Kwaan, Origin of fibrinolytic activity in cultures of human kidney. J. Lab. Clin. Med. 70:650–661, 1967.
M.B. Bernik and H.C. Kwaan, Plasminogen activator activity in cultures from human tissues. An immunological and histochemical study, J. Clin. Invest. 48:1740–1753, 1969.
L.J. Lewis, Plasminogen activator (urokinase) from cultured cells. Thrombo. and Haem. 42:895–900, 1979.
E.F. Howard, C.Y. Cheng and J.S. Howard, Mitogenic activity and plasminogen activator in harvest fluid concentrates from mammary cells in culture. Cancer Res. 40:4385–4389, 1980.
A.J. Johnson, D.L. Kline and N. Alkjaersig, Assay methods and standard preparation for plasmin, plasminogen and urokinase in purified systems, in: “Recommendations of the NHI Committee on Thrombolytic Agents, Subcommittee for Standardization, (F.K. Schallauer, ed.), Verlag., Stuttgart, NY Vol. XXI:259–272 (1969).
W.F. White, G.H. Barlow and M.M. Mozen, The isolation and characterization of plasminogen activators (urokinase) from human urine, Biochem. 5:2160–2169, 1966.
L. Holmberg, B. Bladh and B. Astedt, Purification of urokinase by affinity chromatography, Biochim. Biophys. Acta. 445:215, 1976.
M.E. Soberano, E.F. Ong and A.J. Johnson, The effects of inhibitors on the catalytic conversions of urokinase, Thromb. Res. 9:675, 1976.
M.B. Bernik and E.P. Oller, Increased plasminogen activator (urokinase) in tissue culture after fibrin deposition. J. Clin. Invest. 52:823–834, 1973.
Plough and N.D. Kjeldgaard, Urokinase; an activator of plasminogen from human urine. I. Isolation and properties. Biochem. Biophys. Acta. 24:278–282, 1957.
J. Unkless, K. Dano, G.M. Kellerman and E. Reich, Fibrinolysis associated with oncogenic transformation. Partial purification and characterization of the cell factor, plasminogen activators. J. Biol. Chem. 249:4295–4305, 1974.
P. Brackman, ed., A standardized fibrin plate method and a fibrinolytic assay of plasminogen, in: “Fibrinolysis,“ Scheltema and Holkema, Amsterdam, p. 124 (1967).
W.L. Bigbee, H.B. Weintraub and R.H. Jensen, Sensitive fluorescence assay for urokinase using synthetic peptide 4-methoxy-B-napthylamide substrates. Analyt. Biochem. 88:114–122, 1978.
G. Claeson, P. Friberger, M. Knos and E. Eriksson, Methods for determination of prekallikrenin in plasma, glandular kallikrenín and urokinase, Haemostasis. 7:76–78, 1978.
G.H. Barlow, A. Reuter and I. Tribby, In: “Proteases in Biological Control,” (e. Reich, D.B. Rifkin and E. Shaw, eds.) Cold Spring Harbor Laboratories, NY, pp. 325–331 (1975).
P.S. Moorhead, Harvesting human leucocyte cultures for chromosome cytology. In: “Tissue Culture Methods and Applications,” (P.F. Kruse, Jr. and M.K. Patterson, Jr., eds.) Academic Press, NY, pp. 768–773 (1973).
R.N. Hull, W.R. Cherry, R.M. Huseby and S.A. Clavin, Studies on tissue culture produced plasminogen activator. I. Preliminary observations and the enhancing effect of colchicine and other antimitotic agents, Thromb. Res. 10:669–677, 1977.
M.B. Bernik and H.C. Kwaan, Inhibitors of fibrinolysis in human tissues in culture, Amer. J. Physiol. 221:916–921, 1971.
D.B. Rifkin and R. Pollack, Production of plasminogen activator by established cell lines of mouse origin, J. Cell Biol. 73:47–55, 1977.
I.N. Chou, R.O. Roblin and P.H. Black, Calcium stimulation of plasminogen activator secretion/production by Swiss 3T3 cells, J. Biol. Chem. 252:6256–6259, 1977.
G.H. Barlow, C.W. Francis and V.J. Marder, On the conversion of high molecular weight urokinase to the low molecular weight by plasmin, Submitted, 1981.
M. Nobuhara, M. Sakamaki, H. Ohnishi and Y. Sasuki, A comparative study of high molecular weight urokinase and low molecular weight urokinase, J. Biochem. 90:225–232, 1981.
R.D. Philo and P.J. Gaffney, Assay methodology for urokinase: Its use in assessing the composition of mixtures of high-and low-molecular weight urokinase. Thromb. Res. 21:81–88, 1981.
R.O. Roblin, P.L. Young and T.E. Bell, Concomitant secretion by transformed SVW138-VA13-2RA cells of plasminogen activator(s) and substance(s) which prevent their detection. Biochem. Biophys. Res. Com. 82:165–172, 1978.
D.J. Luskutoff and T.S. Edgington, An inhibitor of plasminogen activator in rabbit endothelial cells, J. Biol. Chem. 256: 4142–4145, 1981.
N. Aoki and T. Kawano, Inhibition of plasminogen activators by naturally occurring inhibitors in man. Amer. J. Physiol. 223:1334–1337, 1972.
G. Wijngaard, Interfering factors in the assay of plasminogen activators by the fibrin plate method. Occurrence of different inhibitors against tissue plasminogen activator and urokinase. Thrombos. Haemostas. 41:590–600, 1979.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Lewis, M.L., Morrison, D.R., Mieszkuc, B.J., Fessler, D.L. (1984). Problems in the Bioassay of Products from Cultured HEK Cells: Plasminogen Activator. In: Acton, R.T., Daniel Lynn, J. (eds) Eukaryotic Cell Cultures. Advances in Experimental Medicine and Biology, vol 172. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9376-8_15
Download citation
DOI: https://doi.org/10.1007/978-1-4615-9376-8_15
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4615-9378-2
Online ISBN: 978-1-4615-9376-8
eBook Packages: Springer Book Archive