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Problems in the Bioassay of Products from Cultured HEK Cells: Plasminogen Activator

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Eukaryotic Cell Cultures

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 172))

Abstract

For research and commercial purposes, the production of materials from cultured cells is progressively becoming routine. Measuring products in cell culture medium, however, is not always straightforward even when the most appropriate product assays are utilized.

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Abbreviations

PA:

plasminogen activator

HEK:

human embryonic kidney

WHO:

World Health Organization

CTA:

Committee on Thrombolytic Agents

HMW:

high molecular weight

LMW:

low molecular weight

CFES:

continuous flow electrophoresis system

References

  1. J.M. Price G.H. Barlow and J.E. Donahoe Mammalian cell culture as a source of biological materials. In “Principles and Techniques of Human Research and Therapeutics A Series of Mographs” F. Gilbert McMahon ed. Futura Publishing company Inc. Mt. Kisko NY XV, 37–54 1978

    Google Scholar 

  2. R. Davis, Technical issues of recombinant DNA in medical products. Presented at Symposium on Medicinals, Chemicals, Diagnostic Products, and Evaluation of New Biotechnology, Stanford Res. Inst., Palo Alto, CA, 1980.

    Google Scholar 

  3. M.B. Bernik and H.C. Kwaan, Origin of fibrinolytic activity in cultures of human kidney. J. Lab. Clin. Med. 70:650–661, 1967.

    Google Scholar 

  4. M.B. Bernik and H.C. Kwaan, Plasminogen activator activity in cultures from human tissues. An immunological and histochemical study, J. Clin. Invest. 48:1740–1753, 1969.

    Article  Google Scholar 

  5. L.J. Lewis, Plasminogen activator (urokinase) from cultured cells. Thrombo. and Haem. 42:895–900, 1979.

    Article  Google Scholar 

  6. E.F. Howard, C.Y. Cheng and J.S. Howard, Mitogenic activity and plasminogen activator in harvest fluid concentrates from mammary cells in culture. Cancer Res. 40:4385–4389, 1980.

    Google Scholar 

  7. A.J. Johnson, D.L. Kline and N. Alkjaersig, Assay methods and standard preparation for plasmin, plasminogen and urokinase in purified systems, in: “Recommendations of the NHI Committee on Thrombolytic Agents, Subcommittee for Standardization, (F.K. Schallauer, ed.), Verlag., Stuttgart, NY Vol. XXI:259–272 (1969).

    Google Scholar 

  8. W.F. White, G.H. Barlow and M.M. Mozen, The isolation and characterization of plasminogen activators (urokinase) from human urine, Biochem. 5:2160–2169, 1966.

    Article  Google Scholar 

  9. L. Holmberg, B. Bladh and B. Astedt, Purification of urokinase by affinity chromatography, Biochim. Biophys. Acta. 445:215, 1976.

    Article  Google Scholar 

  10. M.E. Soberano, E.F. Ong and A.J. Johnson, The effects of inhibitors on the catalytic conversions of urokinase, Thromb. Res. 9:675, 1976.

    Article  Google Scholar 

  11. M.B. Bernik and E.P. Oller, Increased plasminogen activator (urokinase) in tissue culture after fibrin deposition. J. Clin. Invest. 52:823–834, 1973.

    Article  Google Scholar 

  12. Plough and N.D. Kjeldgaard, Urokinase; an activator of plasminogen from human urine. I. Isolation and properties. Biochem. Biophys. Acta. 24:278–282, 1957.

    Article  Google Scholar 

  13. J. Unkless, K. Dano, G.M. Kellerman and E. Reich, Fibrinolysis associated with oncogenic transformation. Partial purification and characterization of the cell factor, plasminogen activators. J. Biol. Chem. 249:4295–4305, 1974.

    Google Scholar 

  14. P. Brackman, ed., A standardized fibrin plate method and a fibrinolytic assay of plasminogen, in: “Fibrinolysis,“ Scheltema and Holkema, Amsterdam, p. 124 (1967).

    Google Scholar 

  15. W.L. Bigbee, H.B. Weintraub and R.H. Jensen, Sensitive fluorescence assay for urokinase using synthetic peptide 4-methoxy-B-napthylamide substrates. Analyt. Biochem. 88:114–122, 1978.

    Article  Google Scholar 

  16. G. Claeson, P. Friberger, M. Knos and E. Eriksson, Methods for determination of prekallikrenin in plasma, glandular kallikrenín and urokinase, Haemostasis. 7:76–78, 1978.

    Google Scholar 

  17. G.H. Barlow, A. Reuter and I. Tribby, In: “Proteases in Biological Control,” (e. Reich, D.B. Rifkin and E. Shaw, eds.) Cold Spring Harbor Laboratories, NY, pp. 325–331 (1975).

    Google Scholar 

  18. P.S. Moorhead, Harvesting human leucocyte cultures for chromosome cytology. In: “Tissue Culture Methods and Applications,” (P.F. Kruse, Jr. and M.K. Patterson, Jr., eds.) Academic Press, NY, pp. 768–773 (1973).

    Chapter  Google Scholar 

  19. R.N. Hull, W.R. Cherry, R.M. Huseby and S.A. Clavin, Studies on tissue culture produced plasminogen activator. I. Preliminary observations and the enhancing effect of colchicine and other antimitotic agents, Thromb. Res. 10:669–677, 1977.

    Article  Google Scholar 

  20. M.B. Bernik and H.C. Kwaan, Inhibitors of fibrinolysis in human tissues in culture, Amer. J. Physiol. 221:916–921, 1971.

    Article  Google Scholar 

  21. D.B. Rifkin and R. Pollack, Production of plasminogen activator by established cell lines of mouse origin, J. Cell Biol. 73:47–55, 1977.

    Article  Google Scholar 

  22. I.N. Chou, R.O. Roblin and P.H. Black, Calcium stimulation of plasminogen activator secretion/production by Swiss 3T3 cells, J. Biol. Chem. 252:6256–6259, 1977.

    Google Scholar 

  23. G.H. Barlow, C.W. Francis and V.J. Marder, On the conversion of high molecular weight urokinase to the low molecular weight by plasmin, Submitted, 1981.

    Google Scholar 

  24. M. Nobuhara, M. Sakamaki, H. Ohnishi and Y. Sasuki, A comparative study of high molecular weight urokinase and low molecular weight urokinase, J. Biochem. 90:225–232, 1981.

    Article  Google Scholar 

  25. R.D. Philo and P.J. Gaffney, Assay methodology for urokinase: Its use in assessing the composition of mixtures of high-and low-molecular weight urokinase. Thromb. Res. 21:81–88, 1981.

    Article  Google Scholar 

  26. R.O. Roblin, P.L. Young and T.E. Bell, Concomitant secretion by transformed SVW138-VA13-2RA cells of plasminogen activator(s) and substance(s) which prevent their detection. Biochem. Biophys. Res. Com. 82:165–172, 1978.

    Article  Google Scholar 

  27. D.J. Luskutoff and T.S. Edgington, An inhibitor of plasminogen activator in rabbit endothelial cells, J. Biol. Chem. 256: 4142–4145, 1981.

    Google Scholar 

  28. N. Aoki and T. Kawano, Inhibition of plasminogen activators by naturally occurring inhibitors in man. Amer. J. Physiol. 223:1334–1337, 1972.

    Article  Google Scholar 

  29. G. Wijngaard, Interfering factors in the assay of plasminogen activators by the fibrin plate method. Occurrence of different inhibitors against tissue plasminogen activator and urokinase. Thrombos. Haemostas. 41:590–600, 1979.

    Article  Google Scholar 

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© 1984 Plenum Press, New York

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Lewis, M.L., Morrison, D.R., Mieszkuc, B.J., Fessler, D.L. (1984). Problems in the Bioassay of Products from Cultured HEK Cells: Plasminogen Activator. In: Acton, R.T., Daniel Lynn, J. (eds) Eukaryotic Cell Cultures. Advances in Experimental Medicine and Biology, vol 172. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9376-8_15

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  • DOI: https://doi.org/10.1007/978-1-4615-9376-8_15

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