Abstract
The IMP dehydrogenase (IMPDH) inhibitor mycophenolic acid (MPA), at concentrations ranging from 5 to 50 nM, induces differentiation in various human neuroblastoma cell lines.1 Purpose of the present study was to investigate guanylate synthesis and cyclic nucleotides metabolism during mycophenolic acid-induced differentiation in the human neuroblastoma cell line LA-N-5.
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Savini F, Rucci C, Messina E, Quaratino CP, Scarpa S, Vasaturo F, Modesti A, and Giacomello A Differentiating and biochemical effects of a reduction of intracellular GTP levels induced by mycophenolic acid (MPA) in human neuroblastoma (NB) cell lines. Adv Exp Med Biol (This volume).
Giacomello A, Salerno C. A continuous spectrophotometric assay for hypoxanthine guanine phosphoribosyltransferase. Anal Biochem, 1977; 79: 263–267.
Atkinson MR, Morton RK, Murray AW. Inhibition of inosine 5′-phosphate dehydrogenase from Ehrlich ascites tumor cells by 6-thioinosine 5′-phosphate. Biochem J, 1963; 89: 167–172.
Spoto G, Whitehead E, Ferraro A, Di Terlizzi PM, Turano C, Riva F. A reverse-phase HPLC method for cAMP phosphodiesterase activity. Anal Biochem, 1991; 196: 207–10.
Di Pierro D, Tavazzi B, Perno CF et al. An ion-pairing high performance liquid Chromatographic method for the direct simultaneous determination of nucleotides, deoxynucleotides, nicotinic coenzymes, oxypurines, nucleosides, and bases in perchloric acid cell extracts. Anal Biochem, 1995; 231: 407–12.
Kiguchi K, Collart FR, Henning-Chubb C, Huberman E. Cell differentiation and altered IMP dehydrogenase expression induced in human T-lymphoblastoid leukemia cells by mycophenolic acid and tiazofurin. Exp Cell Res, 1990; 187: 47–53.
La Rosa FG, Kumar S, Prasad KN Increased expression of ubiquitin during adenosine 3′,5′ monophosphate-induced differentiation of neuroblastoma cells in culture. J Neurochem. 1996; 66: 1845–50.
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Quaratino, C.P. et al. (1998). Imp-Dehydrogenase (IMPDH), Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT), and Phosphodiesterases (PDEs) Expression During Mycophenolic Acid (MPA)-Induced Differentiation in Human Neuroblastoma Cell Lines. In: Griesmacher, A., Müller, M.M., Chiba, P. (eds) Purine and Pyrimidine Metabolism in Man IX. Advances in Experimental Medicine and Biology, vol 431. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5381-6_86
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DOI: https://doi.org/10.1007/978-1-4615-5381-6_86
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