Abstract
Replicons based upon the human coronavirus 229E (HCV 229E) genome were transfected into HCV 229E infected cells. We demonstrate that a synthetic RNA comprised of 646 nucloetides from the 5′ end and 1465 nucloetides from the 3′ end of the HCV 229E genome is replication competent. We conclude that the cis-acting elements necessary for replication are located in these 5′ and 3′ genomic regions. Furthermore, we inserted the intergenic region of the HCV 229E nucleocapsid protein gene into this basic construct and were able to demonstrate the transcription of “subgenomic” RNAs
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Herold, J., Raabe, T., Schelle-Prinz, B., and Siddell. S.G., 1993, Nucleotide sequence of the human coronavirus 229E RNA polymerase locus, Virology 195: 680–691.
Makino, S., and Lai. M.M.C., 1989, High-frequency leader sequence switching during Coronavirus defective interfering RNA replication, J. Virol. 63: 5285–5292.
Makino, S., Joo, M., and Makino. J.K., 1991, A system for study of Coronavirus mRNA synthesis: a regulated, expressed subgenomic defective interfering RNA results from intergenic site insertion, J. Virol. 65: 6031–6041.
Meinkoth, J., and Wahl. G., 1984, Hybridization of nucleic acids immobilized on solid supports, Anal.Biochem. 138: 267–284.
Van der Most, R.G., Bredenbeck, P. J., and Spaan. W.J.M., 1991, A domain at the 3’ end of the polymerase gene is essential for encapsidation of Coronavirus defective interfering RNAs, J. Virol. 65: 3219–3226.
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© 1998 Springer Science+Business Media New York
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Thiel, V., Siddell, S.G., Herold, J. (1998). Replication and Transcription of HCV 229E Replicons. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_14
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DOI: https://doi.org/10.1007/978-1-4615-5331-1_14
Publisher Name: Springer, Boston, MA
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