Abstract
In the last ten years it has become possible to routinely analyse molecules of the size of alcohol dehydrogenase (ADH) by mass spectrometry. This has come about primarily as a result of the introduction of two new ionisation methods, namely matrix assisted laser desorption ionisation (MALDI) (Karas and Hillenkamp, 1988) and electrospray (ES).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Emmett, M. R. and Caprioli, R. M., 1994, Micro-electrospray mass spectrometry: ultra-high-sensitivity analysis of peptides and proteins. J. Am. Soc. Mass Spectrom., 5, 605–613.
Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F. and Whitehouse, C. M., 1989, Electrospray ionization for mass spectrometry of large biomolecules. Science, 246, 64–714.
Gale, D. C. and Smith, R. D., 1993, Small volume and low flow-rate electrospray ionization mass spectrometry of aqueous samples. Rapid Commun. Mass Spectrom., 7, 1017–1021.
James, P., Quadroni, M., Carafoli, E. and Gonnett, G., 1993, Protein identification by mass profile fingerprinting. Biochem. Biophys. Res. Com., 195, 5–8.
Karas, M. and Hillenkamp, F., 1988, Laser desorption ionization of proteins with molecular masses exceeding 10 000 daltons. Anal Chem., 60, 1299–2301.
Kelly, M. A., Murphy, C. M. and Fenselau, C. C, 1991, Evaluation of a vestec electrospray source and a phrasor high energy dynode detector on a Hewlett Packard quadrupole mass spectrometer. In: Proceedings of the 39th Annual Conference on Mass Spectrometry and Allied Topics, Nashville, TN, June 1991. ASMS, East Lansing, MI, 136–137.
Loo, J. A., 1994, Observation of large subunit protein complexes by electrospray ionization mass spectrometry. J. Mass Spectrom., 30, 180–183.
Mann, M., Højrup, P. and Roepstorff, P., 1993, Use of mass spectrometric information for the identification of proteins in sequence databases. Biolog. Mass Spectrom., 22, 338.
Meng, C. K., Mann, M. and Fenn, J. B., 1988, Electrospray ionization of some polypeptides and small proteins. In: Proceedings of the 36th Annual Conference on Mass Spectrometry and Allied Topics, San Francisco, CA, June 1988. ASMS, East Lansing, MI, 711–772.
Pappin, D. J. C, Højrap, P. and Bleasby, A. J., 1993, Rapid identification of proteins by peptide-mass fingerprinting. Current Biology, 3, 327.
Speir, J. P., Senko, M. W., Little, D. P., Loo, J. A. and McLafferty, F. W., 1995, High resolution tandem mass spectra of 36-67 kDa proteins. J. Mass Spectrom., 30, 39–42.
Wilm, M. and Mann, M., 1996, Analytical properties of the nanoelectrospray ion source. Anal. Chem., 68, 1–8.
Yamashita, M. and Fenn, J. B., 1984, Electrospray ion source. Another variation on the free-jet theme. J. Phys. Chem., 88, 4451–4459.
Yates, J. R., Speicher, S., Griffin, P. R. and Hunkapiller, T., 1993, Peptide mass maps: a highly informative approach to protein identification. Anal. Biochem., 214, 397–408.
Yates, J. R., Eng, J. K., Clauser, K. R. and Burlingame, A. L., 1996, Search of sequence data bases with uninter-prited high energy collision-induced dissociation spectra of peptides. J. Am. Soc. Mass Spectrom., 7, 1089–1098.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Griffiths, W.J. (1999). Tandem Mass Spectrometry of Alcohol Dehydrogenase and Related Biomolecules. In: Weiner, H., Maser, E., Crabb, D.W., Lindahl, R. (eds) Enzymology and Molecular Biology of Carbonyl Metabolism 7. Advances in Experimental Medicine and Biology, vol 463. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4735-8_37
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4735-8_37
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7146-5
Online ISBN: 978-1-4615-4735-8
eBook Packages: Springer Book Archive