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Localization of GTP Cyclohydrolase I (GTPCH) mRNA in the Rat Brain by in Situ Hybridization

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Chemistry and Biology of Pteridines and Folates

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 338))

Abstract

GTP cyclohydrolase I (GTPCH) is the first and rate-limiting enzyme in the tetrahydrobiopterin (BH4) biosynthetic pathway. BH4 is the essential cofactor for the pterin-dependent monooxygenases which include tyrosine and tryptophan hydroxylase, enzymes that are rate limiting in the biosynthesis of catecholamines and indolamines. BH4 is also required as a cofactor for the family of nitric oxide synthases1,2. We have obtained a clone for GTPCH based on the recently published full-length cDNA encoding GTPCH from rat liver3,4. The in situ hybridization technique was used to study the cellular localization and the relative levels of expression of GTPCH mRNA in the rat brain.

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Author information

Authors and Affiliations

  1. Cellular and Clinical Neurobiology Program, Department of Psychiatry, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA

    Stephen I. Lentz, Kei Hirayama & Gregory Kapatos

Authors
  1. Stephen I. Lentz
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  2. Kei Hirayama
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  3. Gregory Kapatos
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Editor information

Editors and Affiliations

  1. Dept of Pharmacology College of Medicine, Universily of South Alabama, Mobile, AL, 36688, USA

    June E. Ayling

  2. Dept. of Biochemistry College of Medicine, University of South Alabama, Mobile, AL, 36688, USA

    M. Gopal Nair

  3. College of Medicine, Universily of South Alabama, Mobile, AL, 36688, USA

    Charles M. Baugh

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© 1993 Springer Science+Business Media New York

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Lentz, S.I., Hirayama, K., Kapatos, G. (1993). Localization of GTP Cyclohydrolase I (GTPCH) mRNA in the Rat Brain by in Situ Hybridization. In: Ayling, J.E., Nair, M.G., Baugh, C.M. (eds) Chemistry and Biology of Pteridines and Folates. Advances in Experimental Medicine and Biology, vol 338. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2960-6_33

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  • DOI: https://doi.org/10.1007/978-1-4615-2960-6_33

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6287-6

  • Online ISBN: 978-1-4615-2960-6

  • eBook Packages: Springer Book Archive

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