Abstract
Our overall aim is to identify and characterize basic mechanisms responsible for defective growth control of cancer cells. Much evidence now supports our earlier hypothesis1 that normal cell proliferation is controlled just prior to the sudden onset of DNA synthesis and related biochemical processes. Deregulation is dependent upon a mechanism normally controlling passage of cells through a restriction point located in late G1 phase of the cell cycle. Cyclins, cyclin dependent kinases (cdks), retinoblastoma protein (pRB) and others have been implicated in passage through G1 into S phase. We conclude that deranged expression of cyclins, particularly of cyclin E, contributes to defective growth control of cancer cells.
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References
A.B. Pardee. A restriction point for control of normal animal cell proliferation, Proc. Natl. Acad. Sci., USA. 71: 1286 (1974).
A.B. Pardee. G1 events and regulation of cell proliferation, Science 246: 603 (1989).
J.G. Gudas, J.L. Fridovich-Keil, M.W. Datta, J. Bryan, A.B. Pardee. Characterization of the murine thymidine kinase-encoding gene and analysis of transcription start point heterogeneity, Gene 118: 205 (1992).
Q.-P. Dou, P. Markell, A.B. Pardee. Thymidine kinase transcription is regulated at G1/S phase by a complex that contains retinoblastomalike protein and a cdc2 kinase, Proc. Natl. A.ad. Sci, USA. 89: 3256 (1992).
Q.-P. Dou, A.H. Levin, J. Wang, K. Helin, A.B. Pardee. G1/S regulated complexes containing E2F, P107, and cyclin-dependent kinases bind to the mouse thymidine kinase gene promoter, submitted to J. Biol. Chem.
J.L. Fridovich-Keil, P.J. Markell, J.G. Gudas, A.B. Pardee. DNA sequences required for serum-responsive regulation of expression from the mouse thymidine kinase promoter, Cell Growth and Differentiation 4: 679 (1993).
D.W. Bradley, J.L. Fridovich-Keil, A.B. Pardee. Serum-responsive expression from murine TK gene sequences is disrupted in transformed cells, in preparation.
P.W. Rossow, V.F.G. Riddle, A.B. Pardee. Synthesis of labile, serum-dependent protein in early G1 controls animal cell growth, Proc. Natl. Acad. Sci. USA. 76: 446 (1979).
Q.-P. Dou, A. Levin, S. Zhao, A.B. Pardee. Cyclic E and cyclin A as candidates for the restriction point protein, Cancer Res. 53: 1493 (1993).
K. Keyomarsi, A.B. Pardee. Redundant cyclin overexpression and gene amplification in breast cancer cells, Proc. Natl. Acad. Sci. USA 90: 1112 (1993).
P. Liang, L. Averboukh, K. Keyomarsi, R. Sager, A.B. Pardee. Differential display and cloning of messenger RNAs from human breast cancer vs. mammary epithelial cells, Cancer Res. 52: 6966 (1992).
P. Liang, L. Averboukh, A.B. Pardee. Distribution of eukaryotic mRNAs by means of differential display: refinements and optimization, Nucleic Acids Res. 21: 3269 (1993).
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© 1994 Springer Science+Business Media New York
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Pardee, A.B., Dou, QP., Keyomarsi, K. (1994). Molecules of Deregulated Cell Cycle Control in Cancer. In: Hu, V.W. (eds) The Cell Cycle. GWUMC Department of Biochemistry Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2421-2_35
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DOI: https://doi.org/10.1007/978-1-4615-2421-2_35
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