Abstract
Previously, we demonstrated induction of a unique macrophage prothrombinase, PCA, in MHV-3 infected BALB/cJ mice. By immunologic screening, a clone representing PCA was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a flbrinogen-like protein. Six additional clones were isolated, and one clone, pl 1-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constituitively express musfiblp, but when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrate when musfiblp was expressed from pl 1-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Levy, G.A., Leibowitz, J.L., and Edgington, T.S. The induction of monocyte procoagulant activity by murine hepatitis virus MHV-3 parallels disease susceptibility in mice. J. Exp. Med 1981;154: 1150–1163.
MacPhee, P.J., Dindzans, V.J., Fung, L.S., and Levy, G.A. Acute and Chronic changes in the microcirculation of the liver in inbred strains of mice following infection with mouse hepatitis virus type 3. Hepatology 1985;5:649–660.
Dindzans, V.J., Skamene, E., and Levy, G.A. Susceptibility/resistance to mouse hepatitis virus strain 3 and macrophage procoagulant activity are genetically linked and controlled by two non-H-2 linked genes. J. Immunol. 1986;137,:2355–2360.
Li, C.Y., Fung, L.S., Chung, S., Crow, A., Myers-Mason, N., Phillips, M. J, Leibowitz, J.L., Cole, E., Ottaway, C.A., and Levy G. Monoclonal antiprothrombinase (3D4.3) prevents mortality from Murine Hepatitis Virus (MHV-3) infection. J. Exp. Med. 1992;176:689–697.
Koyama, S., Hall, L.R., Haser, W.G., Tonegawa, S., and Saito, H. Structure of a cytotoxic T-lymphocyte-specific gene shows a strong homology to fibrinogen β and γ chains. Proc. Natl. Acad. Sci. USA. 1987; 84:1609–1613.
Rodriguez, D., Zhou, Y., Rodriguez, J-R., Durbin, R.K., Jimenz, V, McAllister, W.T., and Esteban, M. Regulated expression of nuclear genes by T3 RNA polymerase and lac repressor, using recombinant vaccinia virus vectors. J. Virol. 1990;64(10):4851–4857.
Evans, R., and Kamdar, S.J. Stability of RNA isolated from macrophages depends on the removal of an RNA-degrading activity early in the extraction procedure. Biotechniques 1990;8(4):357–360.
Sambrook, J., Fritsch, E.F., and Maniatis, T. In: Molecular Cloning: A Laboratory Manual, Second Edition (Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press) 1989.
Levy, G.A., and Edgington, T.S. Lymphocyte Cooperation is required for amplification of macrophage procoagulant activity. J. Exp. Med. 1980;151:1232–1244.
Ottaway, C.A., Warren, R. E., Saibil, F.G., Fung, L.S., Fair, D.S., and Levy, G.A. Monocyte procoagulant activity in Whipple’s disease. J. Clin. Immunol. 1984;4(5):348–358.
Fung, L.S., Neil, G., Leibowitz, J., Cole, E.H., Chung, S., Crow, A., and Levy, G.A.. Monoclonal antibody analysis of a unique macrophage procoagulant activity induced by murine hepatitis virus strain 3 infection. J. Biol. Chem. 1991;226(3): 1789–1795.
Chung, S, Sinclair, S., Leibowitz, J, Skamene, E., Fung, L.S., and Levy, G.A. Cellular and metabolic requirements for induction of macrophage procoagulant activity by murine hepatitis virus strain 3 in vitro. J. Immunol. 1991;145:271–278.
Dindzans, V.J., MacPhee, P.J., Fung, L.S., Leibowitz, J.L., and Levy, G.A. The immune response to mouse hepatitis virus: Expression of monocyte procoagulant activity and plasminogen activator during infection in vivo. J. Immunol. 1985;135(6):4189–4197.
Fenton, J.W. Thrombin. Ann. N.Y. Acad. Sci. 1986;485:5–15.
Haver, V.M. and Namm, D.H. Characterization of the thrombin induced contraction of vascular smooth muscle. Blood Vessels 1984;21:53–63.
Jungi, T.W., Spycher, M.O., Nydegger, V.E., and Banandum, S. Platelet-leukocyte interaction: Selective binding of thrombin stimulated platelets to human monocytes, polymorphonuclear leukocytes and related cell lines. Blood 1986;67:629–636.
Levy, G.A., Leibowitz, J., and Edgington, T.S. Lymphocyte-instructed monocyte induction of the coagulation pathways parallels the induction of hepatitis by the murine hepatitis virus. Prog. Liver Dis. 1982; 7: 393–409.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Parr, R.L., Fung, L., Reneker, J., Myers-Mason, N., Leibowitz, J.L., Levy, G. (1995). MHV-3 Induced Prothrombinase is Encoded by MUSFIBLP . In: Talbot, P.J., Levy, G.A. (eds) Corona- and Related Viruses. Advances in Experimental Medicine and Biology, vol 380. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1899-0_25
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1899-0_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5775-9
Online ISBN: 978-1-4615-1899-0
eBook Packages: Springer Book Archive