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Distant Hybridization Involving Different In Vitro Techniques

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Biotechnology of Crucifers

Abstract

The Brassicaceae family has extensive genetic type and variation, which makes distant hybridization to be a potential strategy for integrating important traits such as quality and resistance to diseases and/or pests into the cultivated species from wild species. However, wide cross incompatibility, often leading to fail to obtain hybrids, limits the application of distant hybridization to crucifers’ breeding. Nowadays, with the development of in vitro techniques, it is possible to use these genetic resources by distant hybridization. This chapter reviews the in vitro techniques including somatic hybridization, embryo rescue, microspore embryogenesis, and chromosome doubling that are involved in distant hybridization.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31000678, 31071698, 31170405), the Science and Technology Department of Zhejiang Province (2012C12902-1, 2011R50026-5), China Postdoctoral Science Foundation (2011M501012, 2012T50555), Jiangsu Province Agricultural Scientific and Technological Autonomous Innovation Project (No. CX (10) 463), and National High Technology Research and Development Program of China (2011AA10A206). Weijun Zhou (the corresponding author) is grateful to the 985-Institute of Agrobiology and Environmental Sciences of Zhejiang University for providing convenience in using the experimental equipments.

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Authors and Affiliations

  1. Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China

    Dan Liu Ph.D., Ling Xu Ph.D., Xinxin Geng B.S., Zhenchao Zhang Ph.D., Bing Wang M.S. & Weijun Zhou Ph.D.

  2. Institute of Tobacco Research, Chinese Academy of Agricultural Sciences, Qingdao, Shandong, 266101, China

    Dan Liu Ph.D.

  3. Agricultural Experiment Station, Zhejiang University, Hangzhou, 310058, China

    Yuanfei Zhou Ph.D. & Weijun Zhou Ph.D.

  4. Zhenjiang Agricultural Research Institute, Jurong, Jiangsu, 212400, China

    Zhenchao Zhang Ph.D.

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  1. Dan Liu Ph.D.
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  2. Ling Xu Ph.D.
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  3. Xinxin Geng B.S.
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  4. Yuanfei Zhou Ph.D.
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  5. Zhenchao Zhang Ph.D.
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  6. Bing Wang M.S.
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  7. Weijun Zhou Ph.D.
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Correspondence to Weijun Zhou Ph.D. .

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  1. Science and Technology, Division of Plant Breeding and Genetics, S.K. University of Agricultural, Chatha, 180 003, India

    Surinder Kumar Gupta

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Liu, D. et al. (2013). Distant Hybridization Involving Different In Vitro Techniques. In: Gupta, S. (eds) Biotechnology of Crucifers. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7795-2_3

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