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Lens Differentiation from Embryonic Stem (ES) and Induced Pluripotent Stem (iPS) Cells

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Stem Cell Biology and Regenerative Medicine in Ophthalmology

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

The formation of lens progenitor cells and differentiated lens tissue in cell culture conditions presents a number of experimental challenges, even though lens lineage formation and lens fiber cell differentiation are among the best characterized model systems at both genetic and molecular levels. Lens differentiation from ES cells in vitro appears to be a feasible goal. This chapter describes the significance of using ES and iPS cells for better understanding of embryonic lens development and formation of congenital cataracts. A discussion of how iPS cells can help studies of age-related cataract is also included. The chapter summarizes the current data on lentoid body formation from human and primate ES cells, and the molecular basis of directed differentiation of human ES cells into lens progenitor cells and lentoid bodies. Finally, current gaps in lens research and future directions to address these problems are discussed.

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Acknowledgements

We thank Dr. Louise Wolf for critical suggestions. We are grateful to Dr. Eric Bouhassira and ES cell core facilities at the Ruth L. and Davis S. Gottesman Institute for Stem Cell Research and Regenerative Medicine of the Albert Einstein College of Medicine for their continuous support. Grant support to AC: R01 EY102200, EY014237 and R21 EY020621. The Department of Ophthalmology and Visual Sciences is supported by an unrestricted grant from Research to Prevent Blindness, Inc.

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Authors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, NY, USA

    Ales Cvekl, Yang Jing & Qing Xie

  2. Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA

    Ales Cvekl & Qing Xie

  3. Division of Cardiology, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA

    Ying Yang

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  1. Ales Cvekl
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  2. Ying Yang
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  4. Qing Xie
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Correspondence to Ales Cvekl .

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  1. Edward S. Harkness Eye Institute, Department of Ophthalmology, Columbia University, 160 Fort Washington Avenue, Room 513, New York, 10032, New York, USA

    Stephen H. Tsang

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Cvekl, A., Yang, Y., Jing, Y., Xie, Q. (2013). Lens Differentiation from Embryonic Stem (ES) and Induced Pluripotent Stem (iPS) Cells. In: Tsang, S. (eds) Stem Cell Biology and Regenerative Medicine in Ophthalmology. Stem Cell Biology and Regenerative Medicine. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-5493-9_4

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