Synopsis
Free-living organisms must duplicate their chromosomes before each cell division. To ensure that this process occurs at the correct time in the cell cycle, chromosomal DNA replication is highly regulated. Despite specific mechanistic differences among organisms, all domains of life use similar procedures to duplicate their chromosomes and to make certain that DNA replication occurs at the proper time. As described in this series of entries, enzymes that act as molecular machines function during each of the stages of DNA replication: initiation, elongation, and termination. The examination of these enzymes reveals that they are fascinating and complex, sharing the common feature of coordinating nucleotide binding and/or its hydrolysis with a conformational change. In addition to conformational changes during catalysis, altered conformations in these molecular machines are frequently induced during their interaction with ligands and other macromolecules that then result in...
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Acknowledgments
I thank members of my lab for their support while I wrote. This work is supported by Grant GM090063 from the National Institutes of Health, and by the Michigan Agricultural Experiment Station.
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Kaguni, J.M. (2018). DNA Replication. In: Wells, R.D., Bond, J.S., Klinman, J., Masters, B.S.S. (eds) Molecular Life Sciences. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-1531-2_53
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DOI: https://doi.org/10.1007/978-1-4614-1531-2_53
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