Fluorescence Studies on Biological Membranes

1. Front Matter

   Pages i-xxiii

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2. Biomembrane Structure and Dynamics Viewed by Fluorescence

   • B. Wieb Van der Meer

   Pages 1-53

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3. Dynamic Structure of Membranes and Subcellular Components Revealed by Optical Anisotropy Decay Methods

   • Kazuhiko Kinosita Jr., Akira Ikegami

   Pages 55-88

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4. Principles of Frequency-Domain Fluorescence Spectroscopy and Applications to Cell Membranes

   • Joseph R. Lakowicz

   Pages 89-126

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5. Time-Resolved Fluorescence Depolarization Techniques in Model Membrane Systems Effect of Sterols and Unsaturations

   • Michel Vincent, Jacques Gallay

   Pages 127-158

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6. Fluorescence Polarization to Evaluate the Fluidity of Natural and Reconstituted Membranes

   • Elsie M. B. Sorensen

   Pages 159-191

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7. Fluidity of Thyroid Plasma Membranes

   • Hugo Depauw, Marc De Wolf, Guido Van Dessel, Herwig Hilderson, Albert Lagrou, Wilfried Dierick

   Pages 193-240

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8. Spectroscopic Analysis of the Structure of Bacteriorhodopsin

   • Akira Ikegami, Tsutomu Kouyama, Hisako Urabe, Kazuhiko Kinosita Jr.

   Pages 241-257

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9. Structure and Dynamics of the Liver Microsomal Monoxygenase System

   • Christoph Richter, Josef Gut, Barbara C. Kunz

   Pages 259-279

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10. Fluorescence Studies on Prokaryotic Membranes

    • P. Proulx

    Pages 281-321

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11. The Study of Cytoskeletal Protein Interactions by Fluorescence Probe Techniques

    • Edward Blatt, William H. Sawyer

    Pages 323-361

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12. Fluorescent Probes for the Acetylcholine Receptor Surface Environments

    • Marino Martinez-Carrion, Jeffrey Clarke, Jose-Manuel Gonzalez-Ros, Jose-Carlos Garcia-Borron

    Pages 363-392

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13. Structural Basis and Physiological Control of Membrane Fluidity in Normal and Tumor Cells

    • Wim J. van Blitterswijk

    Pages 393-413

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14. Fusion of Enveloped Viruses with Biological Membranes Fluorescence Dequenching Studies

    • Nor Chejanovsky, Ofer Nussbaum, Abraham Loyter, Robert Blumenthal

    Pages 415-456

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15. Back Matter

    Pages 457-465

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As stated by its first editor, Dr. D. B. Roodyn, the primary goal of the series Subcellular Biochemistry is to achieve an integrated view of the cell by bringing together results from a wide range of different techniques and disciplines. This volume deals with the applications of fluorescence spectroscopy to membrane research. It seeks to present complementary biochemical and bio­ physical data on both the structure and the dynamics of biological membranes. Biophysics and biochemistry are improving more and more in their ability to study biomembranes, overlapping somewhat in this area and explaining the functioning of the whole cell in terms of the properties of its individual com­ ponents. Therefore, we have brought together an international group of experts in order to report on and review advances in fluorescence studies on biological membranes, thereby highlighting subcellular aspects. The first chapters present a critical evaluation of the current applications of dynamic and steady-state fluorescence techniques. Subsequent chapters dis­ cuss more specific applications in cells, biological membranes, and their con­ stituents (lipids, proteins).

• Biomembran
• DNA
• Lipid
• Oxidation
• Polypeptide
• plasma membrane
• proteins
• reductase

• University of Antwerp, Antwerp, Belgium

  H. J. Hilderson

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