In vitro Selection

Abstract

Direct selection of mutant (Gengenbach et al. 1977; Hibberd 1984; Miao et al. 1988; Anderson and Georgeson 1989; Parker et al. 1990; Diedrick et al. 1990) and transgenic (Gordon-Kamm et al. 1990; Fromm et al. 1990) maize tissue cultures is usually conducted using friable, embryogenic callus (Armstrong and Green 1985) or regenerable suspension cultures (Green et al. 1983). Regenerable haploid callus cultures (Coumans et al. 1989; Pescitelli et al. 1989) may allow direct selection of certain recessive mutations as well as negative selection protocols for selection and identification of auxotrophic mutants. The procedures described in this chapter outline direct selection protocols that have been used successfully to select diploid tissue cultures from which mutant and/or transgenic plants have been regenerated.