This paper focuses on conventional and advanced promising methods for the evaluation of toxicity originated by xenobiotics in WWTPs and surface waters. A concise review about toxicity tests applied to activated sludge and to receiving water bodies was presented. Advantages or limitations of the methods were discussed. Both heterotrophic and nitrifying activity were considered. Experimental results on the application to activated sludge of conventional toxicity tests (based on respirometry and bioluminescence) and advanced methods (based on the direct quantification of viable or dead bacteria by using flow cytometry) were presented in this paper. The advantage of respirometry and flow cytometry is related to the use of bacteria present in WWTPs, without need of using pure bacterial strains different from activated sludge.
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References
Amann R.I., Ludwig W., Schleifer K.H. (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59: 143-169.
Dalzell D.J.B., Alte S., Aspichueta E., de la Sota A., Etxebarria J., Gutierrez M., Hoffmann C.C., Sales D., Obst U. and Christofi N. (2002) A comparison of five rapid direct toxicity assessment methods to determine toxicity of pollutants to activated sludge. Chemosphere, 47: 535-545.
Dalzell D.J.B. and Christofi N. (2002) An ATP luminescence method for direct toxicity assessment of pollutants impacting on the activated sewage sludge process. Water Research, 36: 1493-1502.
De Bel M., Stokes L., Upton J. and Watts J. (1996) Applications of a respirometry based toxicity monitor. Wat. Sci. Tech., 33 (1): 289-296.
Devisscher M., Thoeye C., De Gueldre G. and Van de Steene B. (2007) Chapter 3.3: Toxicity evaluation. In: Wastewater Quality Monitoring and Treatment. Edited by P. Quevauviller, O. Thomas, A. Van der Beken. John Wiley & Sons, Ltd.
Farré M. and Barceló D. (2003) Toxicity testing of wastewater and sewage sludge by biosensors, bioassays and chemical analysis. Trends Anal. Chem., 22(5), 299-310.
Franklin N.M., Adams M.S., Stauber J.L. and Lim R.P. (2001) Development of an Improved Rapid Enzyme Inhibition Bioassay with Marine and Freshwater Microalgae Using Flow Cytometry. Arch. Environ. Contam. Toxicol. 40: 469-480.
Gernaey K., Verschuere L., Luyten L. and Verstraete W. (1997) Fast and sensitive acute toxicity detection with an enrichment nitrifying culture. Water Environ. Res., 69(6): 1163-1169.
Guitérrez M., Etxebarria J., De las Fuentes L. (2002) Evaluation of wastewater toxicity: comparative study between Microtox® and activated sludge oxygen uptake inhibition. Water Research, 36: 919-924.
Halling-Sørensen B. (2001) Inhibition of aerobic growth and nitrification of bacteria in sewage sludge by antibacterial agents. Arch. Environ. Contam. Toxicol. 40: 451-460.
ISO (1989) 9509. Method for assessing the inhibition of nitrification of activated sludge microorganisms by chemicals and waste waters. International Organization of Standardization, ISO, Geneva, Switzerland.
ISO (1989) 8692. Water quality-fresh water test with Scendemus subspicatus and Selenastrum Capricornutum. International Organization of Standardization, ISO, Geneva, Switzerland.
ISO (1995) 8192. Water Quality-Test for Inhibition of Oxygen Consumption by Activated Sludge. International Organization of Standardization, ISO, Geneva, Switzerland.
Love N.G. and Bott C.B. (2000). A review and needs survey of upset Early Warning Devices. Final Report Project 99 WWF2. Water Environment Research Fundation.
Nebe-von-Caron G., Stephens P.J., Hewitt C.J., Powell J.R. and Badley R.A. (2000) Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. J. Microbiol. Meth., 42: 97-114.
OECD (1987) OECD 209 method, Activated Sludge, Respiration Inhibition Test, OECD Guidelines Testing of Chemicals, Org. Econ. Cooperat. Dev., Paris, France.
Ren S. (2004) Assessing wastewater toxicity to activated sludge: recent research and developments. Environment International, 30 (8): 1151-1164.
Ricco G., Tomei M.C., Ramadori R. and Laera G. (2004) Toxicity assessment of common xenobiotic compounds on municipal activated sludge: comparison between respirometry and Microtox. Water Research, 38: 2103-2110.
Volskay V.T. Jr. and Grady C.P.L. (1990) Respiration inhibition kinetic analysis. Water Research, 24: 863-874.
Wagner M., Amann R., Lemmer H. and Schleifer K.H. (1993) Probing activated sludge with proteobacteria-specific oligonucleotides: inadequacy of culture-dependent methods for describing microbial community structure. Appl. Environ. Microbiol. 59: 1520-1525.
Ziglio G., Andreottola G., Barbesti S., Boschetti G., Bruni L., Foladori P. and Villa R. (2002) Assessment of activated sludge viability with flow cytometry. Water Research, 36 (2): 460-468.
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Andreottola, G., Foladori, P., Ziglio, G., Cantaloni, C., Bruni, L., Cadonna, M. (2008). Methods For Toxicity Testing Of Xenobiotics In Wastewater Treatment Plants And In Receiving Water Bodies. In: Hlavinek, P., Bonacci, O., Marsalek, J., Mahrikova, I. (eds) Dangerous Pollutants (Xenobiotics) in Urban Water Cycle. NATO Science for Peace and Security Series. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6795-2_18
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DOI: https://doi.org/10.1007/978-1-4020-6795-2_18
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