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Use of Alfalfa In Vitro Cultures in Studies on Regulation of Cyclin-Dependent Kinase (CDK) Functions

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Biotechnology and Sustainable Agriculture 2006 and Beyond

The production of new cells by division and their subsequent elongation are key cellular events in the plant life cycle. The orderly progression of cells through the various phases of the cell cycle and their appropriate responses to nutritional, hormonal, developmental and environmental signals are governed by multiple regulatory mechanisms, including reversible protein phosphorylation, inter- actions of proteins, and specific protein degradation. Since protein phosphorylation is widely recognized to be the major mechanism controlling cell cycle progression, the roles of different kinases, and particularly the family of cyclin-dependent protein kinases (CDKs), are critical for cell division control. After discovery of plant homolo gues of cdc2/CDC28 kinases by complementation of the yeast temp erature sensitive mutants, the functional characterization of the plant CDK genes and protein complexes was largely based on the use of tissue culture systems.

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Dudits, D., Cserháti, M., Miskolczi, P., Fehér, A., Ayaydin, F., Horváth, G.V. (2007). Use of Alfalfa In Vitro Cultures in Studies on Regulation of Cyclin-Dependent Kinase (CDK) Functions. In: Xu, Z., Li, J., Xue, Y., Yang, W. (eds) Biotechnology and Sustainable Agriculture 2006 and Beyond. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6635-1_20

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