Abstract
The isolation of DNA from placental tissue suspected of infection is an important tool for identifying microorganisms such as bacteria, fungi, and viruses associated with complications during and after pregnancy. While experts primarily process placental tissue, the preservation methods employed pose challenges to extracting complete DNA. Therefore, selecting the appropriate protocol is paramount to achieving greater efficiency in obtaining genetic material.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kalmar A, Peterfia B, Wichmann B, Patai AV, Bartak BK, Nagy ZB et al (2015) Comparison of automated and manual DNA isolation methods for DNA methylation analysis of biopsy, fresh frozen, and formalin-fixed, paraffin-embedded colorectal cancer samples. J Lab Autom 20:642–651
Flórez-Vargas O, González C (2004) Amplificación por PCR de tejidos de archivo. Efecto de los fijadores. Salud UIS 36:56–64
Chung JY, Yi JM, Xie R, Brown V, Lee O, Ahuja N et al (2012) A pressure cooking-based DNA extraction from archival formalin-fixed, paraffin-embedded tissue. Anal Biochem 425:128–134
Fox GE, Van Wesep R, Resau JH, Sun CC (1991) The effect of immersion formaldehyde fixation on human placental weight. Arch Pathol Lab Med 115:726–728
Yong HEJ, Chan SY, Chakraborty A, Rajaraman G, Ricardo S, Benharouga M et al (2021) Significance of the placental barrier in antenatal viral infections. Biochim Biophys Acta Mol basis Dis 1867:166244
Arora N, Sadovsky Y, Dermody TS, Coyne CB (2017) Microbial vertical transmission during human pregnancy. Cell Host Microbe 21:561–567
Martinez RM, Wolk DM (2016) Bloodstream infections. Microbiol Spectr 4. https://doi.org/10.1128/microbiolspec.DMIH2-0031-2016
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Villavicencio-Carrisoza, O., Mora-Vargas, C.D., Flores-Villanueva, J., Martínez-Salazar, M.G., Helguera-Repetto, A.C. (2024). Isolation of total DNA from Placenta Samples, both Fresh and following Formalin and Paraffin Treatment. In: Zaga-Clavellina, V. (eds) Maternal Placental Interface. Methods in Molecular Biology, vol 2781. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3746-3_16
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3746-3_16
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3745-6
Online ISBN: 978-1-0716-3746-3
eBook Packages: Springer Protocols