Abstract
Immunophenotyping allows for the deep characterization of leukocytes present in biological samples. Here, we describe a complete procedure for the immunophenotyping of amniotic fluid, which can provide information into the immune processes taking place in the amniotic cavity. The protocol describes amniotic fluid cell count determination, processing, and the use of viability, extracellular antibody, and intracellular/intranuclear antibody staining prior to flow cytometer acquisition.
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Acknowledgments
NGL was supported by the Wayne State University Perinatal Initiative in Maternal, Perinatal and Child Health.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Xu, Y., Miller, D., Galaz, J., Gomez-Lopez, N. (2024). Immunophenotyping of Leukocytes in Amniotic Fluid. In: Zaga-Clavellina, V. (eds) Maternal Placental Interface. Methods in Molecular Biology, vol 2781. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3746-3_14
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DOI: https://doi.org/10.1007/978-1-0716-3746-3_14
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3745-6
Online ISBN: 978-1-0716-3746-3
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