Abstract
The dissociation of whole tissue into single-cell suspensions is a critical step for techniques focused on profiling of individual cells. Here, we describe a protocol for the preparation of high-quality single-cell suspensions from human placental tissues: the basal plate (BP), placental villi (PV), and chorioamniotic membranes (CAM). This protocol also provides guidance for the cryopreservation and recovery of single-cell suspensions for later use. The methods described here have been demonstrated to be suitable for downstream single-cell applications, such as single-cell RNA-sequencing, that require viable, high-quality cell suspensions.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Pique-Regi R, Romero R, Tarca AL et al (2019) Single cell transcriptional signatures of the human placenta in term and preterm parturition. elife 8:e52004
Pique-Regi R, Romero R, Tarca AL et al (2020) Does the human placenta express the canonical cell entry mediators for SARS-CoV-2? elife 9:e58716
Garcia-Flores V, Romero R, Xu Y et al (2022) Maternal-fetal immune responses in pregnant women infected with SARS-CoV-2. Nat Commun 13(1):320
Garcia-Flores V, Xu Y, Pusod E et al (2023) Preparation of single-cell suspensions from the human placenta. Nat Protoc 18(3):732–754
Acknowledgments
NGL was supported by the Wayne State University Perinatal Initiative in Maternal, Perinatal and Child Health.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Garcia-Flores, V., Miller, D., Galaz, J., Gomez-Lopez, N. (2024). Dissociation of Placental Tissues for Single-Cell Techniques. In: Zaga-Clavellina, V. (eds) Maternal Placental Interface. Methods in Molecular Biology, vol 2781. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3746-3_13
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3746-3_13
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3745-6
Online ISBN: 978-1-0716-3746-3
eBook Packages: Springer Protocols