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Imaging of Extravasation of Splenocytes in the Dorsal Skinfold Window Chamber

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Mouse Models of Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2773))

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Abstract

Infiltration of immune cells into the tumor is one of the major drivers of antitumor immune response, which can direct the outcome of anticancer therapies. In mice, implantation of dorsal skinfold window chamber (DSWC) combined with intravital confocal fluorescence microscopy allows real-time observation of splenocyte extravasation and infiltration into tumors. Here, we describe a detailed procedure of the DSWC implantation, splenocyte isolation and fluorescent labeling, intravenous injection of labeled splenocytes, and imaging of splenocyte extravasation into tumors using confocal fluorescence microscopy.

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Acknowledgments

This work was supported by research program P3-0003.

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Authors and Affiliations

  1. Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia

    Tim Bozic & Bostjan Markelc

Authors
  1. Tim Bozic
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  2. Bostjan Markelc
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Corresponding author

Correspondence to Bostjan Markelc .

Editor information

Editors and Affiliations

  1. Institute of Oncology Ljubljana, Ljubljana, Slovenia

    Maja Čemažar

  2. Institute of Oncology Ljubljana, Ljubljana, Slovenia

    Tanja Jesenko

  3. Institute of Oncology Ljubljana, Ljubljana, Slovenia

    Urša Lampreht Tratar

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Bozic, T., Markelc, B. (2024). Imaging of Extravasation of Splenocytes in the Dorsal Skinfold Window Chamber. In: Čemažar, M., Jesenko, T., Lampreht Tratar, U. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 2773. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3714-2_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3714-2_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3713-5

  • Online ISBN: 978-1-0716-3714-2

  • eBook Packages: Springer Protocols

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