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Quantitative Peptidomics: General Considerations

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Peptidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2758))

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Abstract

Peptidomics is the detection and identification of the peptides present in a sample, and quantitative peptidomics provides additional information about the amounts of these peptides. It is possible to perform absolute quantitation of peptide levels in which the biological sample is compared to synthetic standards of each peptide. More commonly, relative quantitation is performed to compare peptide levels between two or more samples. Relative quantitation can measure differences between all peptides that are detectable, which can exceed 1000 peptides in a complex sample. In this chapter, various techniques used for quantitative peptidomics are described along with discussion of the advantages and disadvantages of each approach. A guide to selecting the optimal quantitative approach is provided, based on the goals of the experiment and the resources that are available.

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Acknowledgments

Thanks to Jonathan Sweedler, Lingjun Li, and Michael Schrader for helpful comments.

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Authors and Affiliations

  1. Departments of Molecular Pharmacology and Neuroscience, Albert Einstein College of Medicine, Bronx, NY, USA

    Lloyd D. Fricker

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  1. Lloyd D. Fricker
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Correspondence to Lloyd D. Fricker .

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Editors and Affiliations

  1. Department of Bioengineering Sciences, Weihenstephan-Tr. University of Applied Science, Freising, Germany

    Michael Schrader

  2. Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA

    Lloyd D. Fricker

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Fricker, L.D. (2024). Quantitative Peptidomics: General Considerations. In: Schrader, M., Fricker, L.D. (eds) Peptidomics. Methods in Molecular Biology, vol 2758. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3646-6_5

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  • DOI: https://doi.org/10.1007/978-1-0716-3646-6_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3645-9

  • Online ISBN: 978-1-0716-3646-6

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