Abstract
Thyroid epithelial cells organize as enclosed follicles containing thyroid hormone precursor, iodinated thyroglobulin, with lumina bordered by the cellular apices. Transepithelial transport determines composition of compartmental milieu essential for both prohormone formation and its downstream conversion to thyroxine. Hence, not only do follicular lumina function as storage vessels but also as physiological reaction chambers into which reactive components, together with the proper salts and water, are secreted. Polarized, two-dimensional cultures of pig thyroid epithelia, prepared using established protocols, provide a convenient system for assessing transport processes subserving hormone production. This chapter details established methods for growing and evaluating integrity of primary pig thyroid cultures for downstream analysis of transport and other key physiological functions.
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Acknowledgments
I thank the Department of Anatomy and Physiology at Kansas State University College of Veterinary Medicine for supporting ongoing studies in my laboratory.
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Fong, P. (2024). Cultured Pig Thyroid Follicular Cells: Electrical Evaluation of Epithelial Integrity. In: Baratta, M. (eds) Epithelial Cell Culture. Methods in Molecular Biology, vol 2749. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3609-1_2
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DOI: https://doi.org/10.1007/978-1-0716-3609-1_2
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