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Phage DNA Extraction, Genome Assembly, and Genome Closure

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Bacteriophages

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2738))

Abstract

Bacteriophages, or more simply phages, are currently experiencing a renaissance in life science research for their roles in natural microbial communities, their potential use as antimicrobials, and biotechnological applications. In the modern era, one of the primary steps in phage characterization is obtaining the sequence of the complete genome; this information can be used to determine the relationship of the phage to known phages, predict phage lifestyle, and is a prerequisite for many downstream applications. This protocol describes methods for determining the complete sequence of a double-stranded DNA bacteriophage genome, including DNA extraction from a phage lysate, sending the DNA out to a sequencing service, assembly of the sequence raw reads, and completion of the genome sequence.

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Author information

Authors and Affiliations

  1. Center for Phage Technology, Texas A&M University, College Station, TX, USA

    Justin Boeckman, Mei Liu, Jolene Ramsey & Jason Gill

  2. Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA

    Justin Boeckman & Mei Liu

  3. Department of Biology, Texas A&M University, College Station, TX, USA

    Jolene Ramsey

  4. Department of Animal Science, Texas A&M University, College Station, TX, USA

    Jason Gill

Authors
  1. Justin Boeckman
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  2. Mei Liu
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  3. Jolene Ramsey
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  4. Jason Gill
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Corresponding author

Correspondence to Jason Gill .

Editor information

Editors and Affiliations

  1. School of Veterinary Medicine, Texas Tech University, Amarillo, TX, USA

    Ebenezer Tumban

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Boeckman, J., Liu, M., Ramsey, J., Gill, J. (2024). Phage DNA Extraction, Genome Assembly, and Genome Closure. In: Tumban, E. (eds) Bacteriophages. Methods in Molecular Biology, vol 2738. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3549-0_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3549-0_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3548-3

  • Online ISBN: 978-1-0716-3549-0

  • eBook Packages: Springer Protocols

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