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Stem Cells and Lineage Commitment

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa, Canada

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2736)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Lineage Tracing by Single-Cell Transcriptomics Decoding Dynamics of Lineage Commitment

    • Ping Yu, Lin Cheng
    Pages 1-7

  3. Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin

    • Pooja Flora, Elena Ezhkova
    Pages 9-21

  4. Targeted Gene Silencing by Using GapmeRs in Differentiating Human-Induced Pluripotent Stem Cells (hiPSC) Toward Pancreatic Progenitors

    • Lucas Unger, Luiza Ghila, Simona Chera
    Pages 23-38

  5. Retinoic Acid-Mediated Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells

    • Sangeeta Dutta, Debosree Pal, M. R. S. Rao
    Pages 39-51

  6. Isolation and Functional Analysis of Myoepithelial Cells from Adult Mouse Submandibular Glands

    • Rika Yasuhara, Seya Kang, Rino Tokumasu, Kenji Mishima
    Pages 53-64

  7. Chromosomal Analysis in Lineage-Specific Mouse Hematopoietic Stem Cells and Progenitors

    • Nur Afizah Yusoff, Zariyantey Abd Hamid, Paik Wah Chow, Salwati Shuib, Izatus Shima Taib, Siti Balkis Budin
    Pages 65-76

  8. Easy and Rapid Methods for Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells and Human Umbilical Wharton’s Jelly–Derived Mesenchymal Stem Cells

    • Figen Abatay Sel, Ayse Erol, Mediha Suleymanoglu, Durdane Serap Kuruca, Fatma Savran Oguz
    Pages 77-84

  9. Encapsulation of MSCs in PRP-Derived Fibrin Microbeads

    • Özge Lalegül-Ãœlker, Şükran Åžeker, AyÅŸe Eser Elçin, YaÅŸar Murat Elçin
    Pages 85-93

  10. Assessing Neuronogenic Versus Astrogenic Bias of Neural Stem Cells Via In Vitro Clonal Assay

    • Laura Rigoldi, Antonello Mallamaci
    Pages 95-103

  11. Stem Cell-Based Modeling Protocol for Parkinson’s Disease

    • Babak Arjmand, Shayesteh Kokabi-Hamidpour, Hamid Reza Aghayan, Sepideh Alavi-Moghadam, Rasta Arjmand, Mostafa Rezaei-Tavirani et al.
    Pages 105-114

  12. Standard Operating Procedure for Production of Mouse Brown Adipose Tissue-Derived Mesenchymal Stem Cells

    • Babak Arjmand, Mostafa Rezaei-Tavirani, Sepideh Alavi-Moghadam, Akram Tayanloo-Beik, Mahdi Gholami, Shayesteh Kokabi-Hamidpour et al.
    Pages 115-125

  13. Development and Validation of Type 2 Diabetic Zebrafish Model for Cell-Based Treatments

    • Babak Arjmand, Sepideh Alavi-Moghadam, Shayesteh Kokabi-Hamidpour, Rasta Arjmand, Mostafa Rezaei-Tavirani, Bagher Larijani et al.
    Pages 127-137

  14. Neuromuscular Junction-on-a-Chip for Amyotrophic Lateral Sclerosis Modeling

    • Sepideh Alavi-Moghadam, Shayesteh Kokabi-Hamidpour, Mostafa Rezaei-Tavirani, Bagher Larijani, Rasta Arjmand, Fakher Rahim et al.
    Pages 139-150

  15. Primary Human Leukemia Stem Cell (LSC) Isolation and Characterization

    • Neslihan Meriç, Fatih KocabaÅŸ
    Pages 151-161

  16. GMP-Compliant Mesenchymal Stem Cell-Derived Exosomes for Cell-Free Therapy in Cancer

    • Babak Arjmand, Sepideh Alavi-Moghadam, Mostafa Rezaei-Tavirani, Shayesteh Kokabi-Hamidpour, Rasta Arjmand, Kambiz Gilany et al.
    Pages 163-176

  17. Establishing Brain Tumor Stem Cell Culture from Patient Brain Tumors and Imaging Analysis of Patient-Derived Xenografts

    • Elham Mahmoudian, Arezu Jahani-Asl
    Pages 177-192

  18. An Optimized Protocol for piggyBac-Induced iPSC Generation from hPBMCs by Automatic Electroporation

    • Pelin Kilic, Begum Cosar
    Pages 193-205

  19. Signaling Pathways in Trans-differentiation of Mesenchymal Stem Cells: Recent Advances

    • Vaishak Kaviarasan, Dikshita Deka, Darshini Balaji, Surajit Pathak, Antara Banerjee
    Pages 207-223

  20. Back Matter

    Pages 225-226
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Keywords

About this book

This detailed volume explores a variety of techniques used to study lineage commitment in stem cells. Further elucidation of the process that stem cells undergo on their way to becoming more specified cell types is vital for a more complete understanding of cell biology and overall physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cells and Lineage Commitment: Methods and Protocols serves as an ideal guide for experts and novices in the field of stem cell biology.

Editors and Affiliations

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Stem Cells and Lineage Commitment

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3537-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3536-0Published: 08 November 2023

  • Softcover ISBN: 978-1-0716-3539-1Due: 09 December 2023

  • eBook ISBN: 978-1-0716-3537-7Published: 07 November 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 226

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Stem Cells, Developmental Biology

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