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Enzymatic Digestion and Single Cell Isolation of Peri-implantation Stage Human Trophoblast Cells

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Trophoblasts

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2728))

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Abstract

Recent developments in the in vitro culture of peri-implantation stage human embryos have expanded opportunities to investigate implantation stage human development and trophoblast differentiation in the absence of maternal tissues. Emerging single cell omics analyses have offered researchers new tools to explore unanswered biological questions to new depths. In order to investigate the dynamics of human trophoblast cell differentiation during implantation at the single-cell resolution, efficient cell dissociation approaches of trophoblasts from embryos are necessary. Here, we describe the protocol for extended culture of peri-implantation stage human embryos with enzymatic digestion and manual collection of individual cells for downstream assays.

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Authors and Affiliations

  1. Colorado Centre for Reproductive Medicine, Lone Tree, CO, USA

    Deirdre M. Logsdon, Toshihko Ezashi & Ye Yuan

Authors
  1. Deirdre M. Logsdon
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  2. Toshihko Ezashi
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  3. Ye Yuan
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Corresponding author

Correspondence to Ye Yuan .

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Editors and Affiliations

  1. Department of Pediatrics, McMaster University Medical Centre, Hamilton, ON, Canada

    Sandeep Raha

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Logsdon, D.M., Ezashi, T., Yuan, Y. (2024). Enzymatic Digestion and Single Cell Isolation of Peri-implantation Stage Human Trophoblast Cells. In: Raha, S. (eds) Trophoblasts. Methods in Molecular Biology, vol 2728. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3495-0_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3495-0_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3494-3

  • Online ISBN: 978-1-0716-3495-0

  • eBook Packages: Springer Protocols

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