Abstract
Type I lipoteichoic acid (LTA) is a glycerol phosphate polymer found in the cell envelope of diverse Gram-positive bacteria. The glycerol phosphate backbone is often further decorated with D-alanine and/or sugar residues. Here, we provide details of a 1-butanol extraction and purification method of type I LTA by hydrophobic interaction chromatography. The protocol has been adapted from methods originally described by Fischer et al. (Eur J Biochem 133:523–530, 1983) and further optimized by Morath et al. (J Exp Med 193:393–397, 2001). We also present information on a 2D nuclear magnetic resonance (NMR) analysis method to gain chemical and structural information of the purified LTA material.
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Acknowledgments
The research in the Rismondo laboratory is supported by the German Research Foundation (DFG) grant RI 2920/3-1 and the Gründling laboratory is supported by the Wellcome Trust grant 210671/Z/18/Z/WT.
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Rismondo, J., Gründling, A. (2024). Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy. In: Ton-That, H. (eds) The Bacterial Cell Wall. Methods in Molecular Biology, vol 2727. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3491-2_9
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DOI: https://doi.org/10.1007/978-1-0716-3491-2_9
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