Abstract
Multiplex suspension array technology enables multiple protein analytes to be measured in a single assay. In this chapter, we describe a polystyrene bead-based fluorescent multiplex array for quantitative measurements of specific food allergens. The array uses monoclonal antibodies for allergen detection and purified allergens as reference standards. Each component of the array can be standardized. The array measures up to 17 foods that are regulated in the United States, the European Union, and Japan.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Black, K.R., Filep, S.C., Smith, B.R.E., Chapman, M.D. (2024). Standardization of Food Allergen Measurements Using Multiplex Array Technology. In: Cabanillas, B. (eds) Food Allergens. Methods in Molecular Biology, vol 2717. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3453-0_15
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DOI: https://doi.org/10.1007/978-1-0716-3453-0_15
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