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Thermodynamic Characterization of Nucleic Acid Nanoparticles Hybridization by UV Melting

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RNA Nanostructures

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2709))

Abstract

The advances in nucleic acid nanotechnology have given rise to various elegantly designed structural complexes fabricated from DNA, RNA, chemically modified RNA strands, and their mixtures. The structural properties of NA nanoparticles (NANP) generally dictate and significantly impact biological function; and thus, it is critical to extract information regarding relative stabilities of the different structural forms. The adequate stability assessment requires knowledge of thermodynamic parameters that can be empirically derived using conventional UV-melting technique. The focus of this chapter is to describe methodology to evaluate thermodynamic data of NANPs complexation based on DNA 12 base-pair (bp) duplex formation as an example.

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Acknowledgments

This work was supported by NIH grant 1 R15EB031388-01 to E.F.K.

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Authors and Affiliations

  1. Chemistry Department, Ball State University, Muncie, IN, USA

    Megan Teter, Ross Brumett, Abigail Coffman & Emil F. Khisamutdinov

Authors
  1. Megan Teter
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  2. Ross Brumett
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  3. Abigail Coffman
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  4. Emil F. Khisamutdinov
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Corresponding author

Correspondence to Emil F. Khisamutdinov .

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Editors and Affiliations

  1. Department of Chemistry, UNC Charlotte, Charlotte, NC, USA

    Kirill A. Afonin

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Teter, M., Brumett, R., Coffman, A., Khisamutdinov, E.F. (2023). Thermodynamic Characterization of Nucleic Acid Nanoparticles Hybridization by UV Melting. In: Afonin, K.A. (eds) RNA Nanostructures. Methods in Molecular Biology, vol 2709. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3417-2_9

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  • DOI: https://doi.org/10.1007/978-1-0716-3417-2_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3416-5

  • Online ISBN: 978-1-0716-3417-2

  • eBook Packages: Springer Protocols

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