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Reverse Transfection of Functional RNA Rings into Cancer Cells Followed by in Vitro Irradiation

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RNA Nanostructures

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2709))

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Abstract

RNA nanoparticles are promising therapeutic platforms to improve radiotherapy since they can be functionalized with multiple small interfering RNAs (RNAi) to simultaneously silence critical radioresistance genes. Here we describe the transfer of RNA rings to mammalian cancer cells through reverse transfection, followed by in vitro irradiation and biological assays as surrogates’ endpoints for radiotherapy efficacy.

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Acknowledgments

This research was also supported in part by a FAPESP-USP SPRINT grant from The Graduate School at the University of North Carolina at Charlotte and Sao Paulo Research Foundation (FAPESP) under FAPESP Grants 2017/50029-6 and CNPq grants 426714/2016-0 and 305700/2017-0 (to R.C.) and a CAPES fellowship (to I.N.F). The authors would also like to thank Mara de Souza Junqueira, MSc, for performing cell irradiation at the University of São Paulo.

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Authors and Affiliations

  1. Comprehensive Center for Precision Oncology, Centro de Investigação Translacional em Oncologia (LIM24), Departamento de Radiologia e Oncologia, Faculdade de Medicina da Universidade de São Paulo and Instituto do Câncer do Estado de São Paulo, São Paulo, SP, Brazil

    Renata de Freitas Saito, Isabella Nevoni Ferreira, Maria Cristina Rangel & Roger Chammas

Authors
  1. Renata de Freitas Saito
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  2. Isabella Nevoni Ferreira
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  3. Maria Cristina Rangel
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  4. Roger Chammas
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Corresponding author

Correspondence to Roger Chammas .

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Editors and Affiliations

  1. Department of Chemistry, UNC Charlotte, Charlotte, NC, USA

    Kirill A. Afonin

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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de Freitas Saito, R., Ferreira, I.N., Rangel, M.C., Chammas, R. (2023). Reverse Transfection of Functional RNA Rings into Cancer Cells Followed by in Vitro Irradiation. In: Afonin, K.A. (eds) RNA Nanostructures. Methods in Molecular Biology, vol 2709. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3417-2_18

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  • DOI: https://doi.org/10.1007/978-1-0716-3417-2_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3416-5

  • Online ISBN: 978-1-0716-3417-2

  • eBook Packages: Springer Protocols

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