Abstract
The goal of protein purification is to separate a specific protein from all other biomolecules. Classical chromatographic procedures have been designed to exploit particular distinguishing features of individual target proteins, such as size, shape, physicochemical properties, and binding affinity. Advances in molecular biology and bioinformatics have positively contributed at every level to the challenge of purifying individual proteins and more recently have led to the development of high-throughput proteomic platforms. In this chapter, a synopsis of advancements in the field of protein chromatography is presented, with reference to the principal tools and resources that are available to assist with protein purification strategies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Sun PD, Foster CE, Boyington JC (2004) Overview of protein structural and functional folds. Curr Protoc Protein Sci Chapter 17:Unit 17.1
Kang TS, Kini RM (2009) Structural determinants of protein folding. Cell Mol Life Sci 66:2341–2361
Bartlett AI, Radford SE (2009) An expanding arsenal of experimental methods yields an explosion of insights into protein folding mechanisms. Nat Struct Mol Biol 16:582–588
Joseph AP, de Brevern AG (2014) From local structure to a global framework: recognition of protein folds. J R Soc Interface 11(95):20131147
Strandberg B (2009) Building the ground for the first two protein structures: myoglobin and haemoglobin. J Mol Biol 392:2–10
Cohn EJ, Strong LE, Hughes WL, Mulford DJ, Ashworth JN, Melin M, Taylor HL (1946) Preparation and properties of serum and plasma proteins. IV. A system for the separation into fractions of the proteins and lipoprotein components of biological tissues and fluids. J Am Chem Soc 68:459–475
Wang K, Huang C, Nice E (2014) Recent advances in proteomics: towards the human proteome. Biomed Chromatogr 28:848–857
Armirotti A, Damonte G (2010) Achievements and perspectives of top-down proteomics. Proteomics 10(20):3566–3576
Whittaker JW (2013) Cell free protein synthesis: the state of the art. Biotechnol Lett 35(2):143–152
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Walls, D., Cooney, G., Loughran, S.T. (2023). A Synopsis of Proteins and Their Purification. In: Loughran, S.T., Milne, J.J. (eds) Protein Chromatography. Methods in Molecular Biology, vol 2699. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3362-5_1
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3362-5_1
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3361-8
Online ISBN: 978-1-0716-3362-5
eBook Packages: Springer Protocols