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Quantitative Spatio-temporal Analysis of Phagosome Maturation in Live Cells

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Phagocytosis and Phagosomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2692))

Abstract

Phagocytosis and phagosome maturation are central processes to the development of the innate and adaptive immune response. Phagosome maturation is a continuous and dynamic process that occurs rapidly. In this chapter we describe fluorescence-based live cell imaging methods for the quantitative and temporal analysis of phagosome maturation of beads and M. tuberculosis as two phagocytic targets. We also describe simple protocols for monitoring phagosome maturation: the use of the acidotropic probe LysoTracker and analyzing the recruitment of EGFP-tagged host proteins by phagosomes.

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Acknowledgments

We thank the host-pathogen interactions in tuberculosis laboratory for useful discussions and comments on the manuscript. This work was supported by the Francis Crick Institute (to MGG), which receives its core funding from Cancer Research UK (FC001092), the UK Medical Research Council (FC001092), and the Wellcome Trust (FC001092). This project has received funding from the European Research Council [31] under the European Union’s Horizon 2020 research and innovation program (grant agreement n° 772022).

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Authors and Affiliations

  1. Host-Pathogen Interactions in Tuberculosis Laboratory, The Francis Crick Institute, London, UK

    Patricia Rosell Arévalo, Beren Aylan & Maximiliano G. Gutierrez

Authors
  1. Patricia Rosell Arévalo
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  2. Beren Aylan
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  3. Maximiliano G. Gutierrez
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Correspondence to Maximiliano G. Gutierrez .

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  1. Department of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada

    Roberto J. Botelho

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Arévalo, P.R., Aylan, B., Gutierrez, M.G. (2023). Quantitative Spatio-temporal Analysis of Phagosome Maturation in Live Cells. In: Botelho, R.J. (eds) Phagocytosis and Phagosomes. Methods in Molecular Biology, vol 2692. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3338-0_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3338-0_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3337-3

  • Online ISBN: 978-1-0716-3338-0

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