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Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis

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Flower Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2686))

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Abstract

The plant seed is a remarkable structure that represents the single most important energy source in global diets. The stages of reproductive growth preceding seed formation are particularly important since they influence the number, size, and quality of seed produced. The progenitor of the seed is the ovule, a multicellular organ that produces a female gametophyte while maintaining a range of somatic ovule cells to protect the seed and ensure it receives maternal nourishment. Ovule development has been well characterized in Arabidopsis using a range of molecular, genetic, and cytological assays. These can provide insight into the mechanistic basis for ovule development, and opportunities to explore its evolutionary conservation. In this chapter, we describe some of these methods and tools that can be used to investigate early ovule development and cell differentiation.

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Author information

Authors and Affiliations

  1. School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, Australia

    Dayton C. Bird, Chao Ma, Weng Herng Leong & Matthew R. Tucker

  2. LAQV REQUIMTE, Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, Porto, Portugal

    Sara Pinto

Authors
  1. Dayton C. Bird
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  2. Chao Ma
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  3. Sara Pinto
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  4. Weng Herng Leong
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  5. Matthew R. Tucker
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Corresponding author

Correspondence to Matthew R. Tucker .

Editor information

Editors and Affiliations

  1. Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain

    José Luis Riechmann

  2. Instituto de Biología Molecular y Celular de Plantas CSIC-UPV, Valencia, Spain

    Cristina Ferrándiz

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Bird, D.C., Ma, C., Pinto, S., Leong, W.H., Tucker, M.R. (2023). Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis. In: Riechmann, J.L., Ferrándiz, C. (eds) Flower Development . Methods in Molecular Biology, vol 2686. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3299-4_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3299-4_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3298-7

  • Online ISBN: 978-1-0716-3299-4

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