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A Simple Ca2+-Imaging Approach of Network-Activity Analyses for Human Neurons

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Stem Cell-Based Neural Model Systems for Brain Disorders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2683))

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Abstract

Rapid advances in light microscopy and development of all-optical electrophysiological imaging tools have greatly leveraged the speed and the depth of neurobiology studies. Calcium imaging is a common method that is useful for measuring calcium signals in cells and has been used as a functional proxy for neuronal activity. Here I describe a simple, stimulation-free approach that measures neuronal network activity and single-neuron dynamics in human neurons. This protocol provides the experimental workflow that includes step-wise illustrations of sample preparations, data processing, and analyses that can be used for quick phenotypical assessment and serves as a quick functional readout for mutagenesis or screen effort for neurodegenerative studies.

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Author information

Authors and Affiliations

  1. Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA

    Zijun Sun

  2. Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA, USA

    Zijun Sun

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  1. Zijun Sun
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Correspondence to Zijun Sun .

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Editors and Affiliations

  1. Department of Molecular Biology, Cell Biology, and Biochemistry, Center for Translational Neuroscience, Carney Institute for Brain Science and Brown Institute of Translational Science, Brown University, Providence, RI, USA

    Yu-Wen Alvin Huang

  2. Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, USA

    ChangHui Pak

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Sun, Z. (2023). A Simple Ca2+-Imaging Approach of Network-Activity Analyses for Human Neurons. In: Huang, YW.A., Pak, C. (eds) Stem Cell-Based Neural Model Systems for Brain Disorders. Methods in Molecular Biology, vol 2683. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3287-1_20

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  • DOI: https://doi.org/10.1007/978-1-0716-3287-1_20

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3286-4

  • Online ISBN: 978-1-0716-3287-1

  • eBook Packages: Springer Protocols

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